鼻咽癌组织中DNA损伤与EB病毒感染的相关性研究

发布时间：2018-03-22 01:07

本文选题：γ-HAX　切入点：EB病毒　出处：《细胞与分子免疫学杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的分析鼻咽癌(NPC)组织磷酸化组蛋白H2AX(γ-H2AX)的表达水平和EB病毒(EBV)的感染情况,探寻二者的相关性。并用细胞实验进行验证,以阐明EBV诱导DNA损伤应答进而促进NPC发生发展的可能机制。方法选取NPC标本50例和鼻咽炎(NPI)20例,采用免疫组化法检测γ-H2AX及EB病毒潜伏膜蛋白1(LMP1)的表达,对LMP1阴性标本采用原位杂交方法检测EB病毒编码的RNA(EBER);采用Western blot法检测EBV感染鼻咽癌细胞CNE1后γ-H2AX表达的变化。结果NPC组γ-H2AX的阳性率达94%,显著高于NPI组的40%;EBV阳性率为94%,显著高于NPI组的30%;97.9%EBV感染的NPC组织γ-H2AX阳性,二者表达有相关性(P0.05)。通过Western blot法检测进一步验证,EBV感染可使CNE1中γ-H2AX的表达量增高。结论γ-H2AX表达和EB病毒感染有密切关联性,EBV感染可能是通过诱导细胞DNA损伤,造成基因组不稳定从而促进NPC的发生发展。
[Abstract]:Objective to analyze the expression level of phosphorylated histone H2AX (纬 -H2AX) and the infection of EBV in nasopharyngeal carcinoma (NPC), and to explore the correlation between them. Methods the expression of 纬 -H2AX and Epstein-Barr virus latent membrane protein (1LMP1) was detected by immunohistochemical method in 50 cases of NPC and 20 cases of nasopharyngitis in order to elucidate the possible mechanism of DNA damage response induced by EBV and promote the development of NPC. The expression of 纬 -H2AX in CNE1 cells infected with LMP1 was detected by Western blot. Results the positive rate of 纬 -H2AX in NPC group was 94 and significantly higher than that in NPI group. The positive rate of 纬 -H2AX in 97.9 V infected NPC tissue was significantly higher than that in NPI group. The expression of 纬 -H2AX was correlated with the expression of 纬 -H2AX by Western blot assay. Conclusion the expression of 纬 -H2AX is closely related to the infection of EB virus. The results suggest that the expression of 纬 -H2AX and EBV may be induced by DNA damage. Cause genomic instability and promote the development of NPC.
【作者单位】：四川大学口腔疾病研究国家重点实验室四川大学华西口腔医院;
【基金】：国家自然科学基金青年基金(30901689);国家自然科学基金(81172579) 四川省科技厅科技支撑计划项目(2013SZ0039)
【分类号】：R739.63

【相似文献】