VEGF在伴鼻息肉的慢性鼻-鼻窦炎中的表达及克拉霉素对其的调节作用

发布时间：2018-03-22 03:14

本文选题：慢性鼻-鼻窦炎　切入点：血管内皮生长因子　出处：《临床耳鼻咽喉头颈外科杂志》2014年05期 　论文类型：期刊论文

【摘要】：目的:探讨血管内皮生长因子(VEGF)在伴鼻息肉的慢性鼻-鼻窦炎(CRSwNP)患者鼻黏膜中的表达及克拉霉素对其的调控作用。方法:收集16例CRSwNP组织及21例单纯鼻中隔偏曲患者下鼻甲黏膜,采用实时定量RT-PCR方法检测样本中VEGF的表达。另外收集10例伴鼻息肉的慢性鼻窦炎鼻息肉组织及5例单纯鼻中隔偏曲患者下鼻甲黏膜,采用体外组织块培养及ELISA方法,观察克拉霉素对VEGF表达的调节作用。结果:①VEGF mRNA在CRSwNP组的表达较对照组增高,差异有统计学意义(P0.01);②经克拉霉素刺激前后比较,CRSwNP组织中VEGF蛋白的表达量下降,差异有统计学意义(P0.05)。结论:CRSwNP组织中VEGF的表达显著升高,推测VEGF在鼻息肉的发病机制中具有极其重要的作用。克拉霉素可能通过抑制VEGF的表达而达到治疗慢性鼻-鼻窦炎的作用。
[Abstract]:Objective: to investigate the expression of vascular endothelial growth factor (VEGF) in the nasal mucosa of chronic rhinosinusitis with nasal polyps and the regulatory effect of clarithromycin on it. Methods: 16 cases of CRSwNP and 21 cases of simple deviation of nasal septum were collected. Patients with inferior turbinate mucosa, The expression of VEGF was detected by real-time quantitative RT-PCR. The mucosa of inferior turbinate was collected from 10 cases of chronic sinusitis and nasal polyp with nasal polyps and 5 cases of simple deviation of nasal septum. Tissue mass culture and ELISA method were used in vitro. Results the expression of mRNA in CRSwNP group was higher than that in control group, and the difference was statistically significant before and after clarithromycin stimulation. Compared with the control group, the expression of VEGF protein in CRSwNP tissues decreased significantly. Conclusion the expression of VEGF is significantly increased in the tissues of VEGF. It is speculated that VEGF plays an extremely important role in the pathogenesis of nasal polyps. Clarithromycin may play a role in the treatment of chronic rhinosinusitis by inhibiting the expression of VEGF.
【作者单位】：华中科技大学同济医学院附属同济医院耳鼻咽喉-头颈外科;
【基金】：国家自然科学基金(No:81020108018)
【分类号】：R765.41

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