茶黄素对葡萄糖诱导的人晶状体上皮细胞一氧化氮合成的影响

发布时间：2018-03-24 12:19

本文选题：茶黄　切入点：葡萄糖　出处：《湖南农业大学学报(自然科学版)》2014年04期

【摘要】：为探明茶黄素 3,3' 双没食子酸酯(TFDG)对人晶状体上皮细胞SRA01/04(Human lens epithelial cells,HLECs SRA01/04)的保护作用,采用MTT法建立高浓度葡萄糖(HG)诱导的HLECs损伤模型,同时观察HLECs细胞形态的变化。用不同浓度(4、8、16、64、32、64、128、256、512μmol/L)TFDG处理HLECs损伤细胞,采用化学比色法和硝酸还原法测定HLECs内一氧化氮(NO)的含量和一氧化氮合酶(NOS)的活性。结果表明:高浓度葡萄糖可以抑制人晶状体上皮细胞生长,用于HLECs损伤模型诱导的最佳葡萄糖浓度为50 mmol/L,最佳诱导时间为24 h;与未经处理的模型细胞相比,经浓度为16~64μmol/L TFDG处理的模型细胞的存活率显著提高(P0.05);各个浓度的TFDG能极显著降低模型细胞内NOS的活性,极显著减少NO的合成(P0.01),且呈浓度依赖性。以上结果表明,TFDG对高浓度葡萄糖诱导的HLECs细胞损伤具有较好的保护作用。
[Abstract]:In order to investigate the protective effect of theaflavin (TFDG) on human lens epithelial cells (SRA01/04(Human lens epithelial cells), the HLECs damage model induced by high concentration glucose was established by MTT method. At the same time, the morphological changes of HLECs cells were observed. HLECs damaged cells were treated with different concentrations of HLECs cells. The content of nitric oxide (no) and the activity of nitric oxide synthase (NOS) in HLECs were determined by chemical colorimetry and nitric acid reduction. The results showed that high concentration of glucose could inhibit the growth of human lens epithelial cells. The optimal glucose concentration for HLECs injury model was 50 mmol / L and the optimal induction time was 24 h. The survival rate of the model cells treated with 16 渭 mol/L TFDG was significantly increased, and the activity of NOS in the model cells was significantly decreased by each concentration of TFDG. The results showed that TFDG could protect HLECs cells induced by high concentration of glucose.
【作者单位】：国家植物功能成分利用工程技术研究中心;湖南农业大学茶学教育部重点实验室;湖南省植物功能成分利用协同创新中心;
【基金】：湖南省科学技术厅项目(2013FJ4231)
【分类号】：R776.1

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