E-钙粘附蛋白在视网膜母细胞瘤的表达及意义

发布时间：2018-03-25 23:24

本文选题：视网膜母细胞瘤　切入点：E-钙粘附蛋白　出处：《中南大学》2011年硕士论文

【摘要】：目的：探讨E-钙粘附蛋白在28例视网膜母细胞瘤肿瘤组织中的表达情况及与临床病理特征之间的相关性。方法：应用免疫组织链霉菌抗生物素蛋白-过氧化物酶连结化学染色法(streptavidin-perosidase, SP法)检测28例视网膜母细胞瘤组织及7例正常视网膜组织中E-钙粘附蛋白的表达；利用SPSS13.0统计分析软件处理,并比较E-钙粘附蛋白在正常视网膜组织和RB组织中的表达,分析E-钙粘附蛋白表达与RB病例临床病理特征的联系以及RB组织中E-钙粘附蛋白表达分级与肿瘤分期、病理分级、是否侵犯视神经和预后的相关性。结果： 1 E-钙粘附蛋白在视网膜母细胞瘤中的弱阳性表达率42.8%(12/28)高于其在正常视网膜组织的弱阳性表达率0%(0/7)；.E-钙粘附蛋白在视网膜母细胞瘤中的强阳性表达率35.7%(10/28)低于其在正常视网膜组织的强阳性表达率85.7%(6/7)。 2按照视网膜细胞瘤病理分型(低分化型和高分化型),肿瘤分期(眼内期和眼外期),复发与初发,是否视神经浸润分组,E-钙粘附蛋白的弱阳性表达率的在两组之间差异(低分化型高于高分化型,眼内期低于眼外期,复发组高于初发组,有视神经浸润高于无视神经浸润组)均有统计学意义(P0.05),与患者年龄、性别、预后无关(P0.05)。 3 E-钙粘附蛋白阳性表达程度与视网膜母细胞瘤的肿瘤分期呈负相关(r=-0.472 p0.01),与视神经的浸润和转移,病理分级呈正相关(r=0.472p0.01,r=0.509p0.01),与预后无明显相关性(r=-0.312P0.01)。结论：E-钙粘附蛋白的表达下降与视网膜母细胞瘤视神经浸润,肿瘤的转移,分化,肿瘤的分型,肿瘤的复发有密切的关系,所以检测E-钙粘附蛋白在视网膜母细胞瘤组织中的表达对判断及预测视网膜母细胞瘤的恶性程度与转移有一定的意义。
[Abstract]:Objective: to investigate the expression of E-cadherin in 28 cases of retinoblastoma and its correlation with clinicopathological features. Methods: immunohistochemical staining of streptavidin-perosidase (SP) was used to detect the expression of E-cadherin in 28 cases of retinoblastoma and 7 cases of normal retina. The expression of E-cadherin in normal retina and RB was compared by SPSS13.0 software. To analyze the relationship between the expression of E-cadherin and the clinicopathological features of RB, and the correlation between the expression of E-cadherin and tumor stage, pathological grade, optic nerve invasion and prognosis. Results:. (1) the weak positive rate of E-cadherin in retinoblastoma was 42.812 / 28) higher than that in normal retinal tissue. The strong positive rate of E-cadherin in retinoblastoma was 35.710 / 28) lower than that in normal retinal tissue. The strong positive expression rate of normal retina was 85.7%. 2 according to the pathological classification of retinoma (low differentiation type and high differentiation type, tumor stage (intraocular phase and extraocular stage), recurrence and initial stage, the difference between the two types was observed. The difference of weak positive expression rate of E-cadherin between the two groups (low differentiation type is higher than high differentiation type, intraocular phase is lower than extraocular phase, recurrence group is higher than primary group. The optic nerve infiltration was higher in the group with optic nerve invasion than that in the group without optic nerve invasion (P 0.05), and had no correlation with age, sex and prognosis (P 0.05). 3 the positive expression of E-cadherin was negatively correlated with the tumor stage of retinoblastoma, and was positively correlated with the invasion and metastasis of optic nerve, and the pathological grade was positively correlated with the prognosis of retinoblastoma. There was no significant correlation between the expression of E-cadherin and the prognosis of retinoblastoma. Conclusion the decrease of the expression of E-cadherin in retinoblastoma is closely related to optic nerve invasion, metastasis, differentiation, classification and recurrence of retinoblastoma. Therefore, the expression of E-cadherin in retinoblastoma is of significance in judging and predicting the malignancy and metastasis of retinoblastoma.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.7

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