鼻息肉中IL-5mRNA和蛋白的定量分析

发布时间：2018-03-26 12:26

本文选题：鼻息肉　切入点：白细胞介素-　出处：《临床耳鼻咽喉头颈外科杂志》2014年14期

【摘要】：目的:检测IL-5mRNA和蛋白在鼻息肉和下鼻甲黏膜中的表达,并探讨其在鼻息肉发生发展中的意义及其表达与鼻息肉临床特征之间的关系。方法:采用实时荧光定量PCR(RT-qPCR)和免疫组织化学方法检测24例鼻息肉(息肉组)和15例下鼻甲黏膜(对照组)中IL-5mRNA定量表达和蛋白的表达水平。结果:鼻息肉组织中IL-5mRNA的表达是下鼻甲黏膜组织的7.52倍(P0.01)。IL-5蛋白在鼻息肉组织阳性表达率为79.17%,显著高于鼻下鼻甲黏膜中的阳性表达率26.67%(χ2=10.52,P0.01)。鼻息肉中组织中,IL-5mRNA的表达在性别、单双侧,初发复发之间差别无统计学意义,但在单发与多发、伴过敏性鼻炎或(和)哮喘与不伴过敏性鼻炎和哮喘之间差异有统计学意义。IL-5蛋白的表达在性别、单双侧,初发复发、伴过敏性鼻炎或(和)哮喘与不伴过敏性鼻炎和哮喘之间差别无统计学意义,但在单发与多发之间差别有统计学意义。结论:IL-5mRNA和蛋白在息肉组织中的高表达,可能是促进鼻息肉发生发展的一个重要因素;IL-5在鼻息肉中的表达高低与部分临床特征有关。
[Abstract]:Objective: to detect the expression of IL-5mRNA and protein in nasal polyps and inferior turbinate mucosa. To explore the significance of its expression in the development of nasal polyps and its relationship with the clinical features of nasal polyps. Methods: 24 cases of nasal polyps (polyp group) and 15 cases of nasal polyps (polyp group) were detected by real-time fluorescence quantitative PCRRT qPCR and immunohistochemical method. Results: the expression of IL-5mRNA in nasal polyps was 7.52 times higher than that in inferior turbinate mucosa. The positive rate of IL-5 protein in nasal polyps was 79.17, which was significantly higher than that in nasal polyps. The positive expression rate in inferior turbinate mucosa was 26.67. The expression of IL-5 mRNA in nasal polyps was found in sex. There was no significant difference between unilateral and bilateral, primary recurrence, but the expression of IL-5 protein was significantly different between single and multiple, allergic rhinitis or / and asthma with and without allergic rhinitis and asthma. There was no significant difference between allergic rhinitis or / or asthma and non-allergic rhinitis and asthma, but there was significant difference between single rhinitis and multiple rhinitis. Conclusion there is a high expression of IL-5 mRNA and protein in polyps. The expression of IL-5 in nasal polyps may be an important factor to promote the development of nasal polyps.
【作者单位】：扬州市江都人民医院耳鼻咽喉科;苏北人民医院临床实验中心;南通大学附属医院耳鼻咽喉科;扬州市江都人民医院病理科;
【基金】：江苏省社会发展基金资助项目(No:2010697) 扬州大学临床医学院资助项目(No:201041)
【分类号】：R765.25

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