自制人血清中期角膜保存液对兔角膜的保存效果

发布时间：2018-03-27 23:13

本文选题：角膜保存液　切入点：人血清　出处：《第三军医大学学报》2011年06期

【摘要】：目的研制一种适合我国国情的人血清角膜中期保存液。方法配制人血清浓度分别为10%(A组)、20%(B组)、30%(C组)、0%(D组)的角膜保存液保存兔角膜,用Optisol-GS角膜保存液作对照组,分别在保存第3、7、10、14天进行角膜内皮细胞活性率检测、HE染色、扫描电镜超微结构观察,并检测角膜保存液的理化性质及生物安全性。结果兔角膜保存至第14天时,C组角膜内皮细胞活性率与对照组比较差异无明显统计学意义(P0.05),C组被保存的角膜组织病理学和超微结构与对照组Optisol-GS比较无显著差异。各组血清角膜保存液理化性质稳定,无生物危险性。D、A、B组与对照组比较,角膜内皮细胞活性率分别在保存第3、7、10天时差异具有明显的统计学意义(P0.05);角膜组织病理学比较,在保存第7天,各实验组与对照组无明显差异,随着时间延长,D、A、B组先后依次出现组织病理学改变;超微结构比较,在保存第7天时,D组与C组及对照组具有明显差异。结论 30%浓度的人血清角膜保存液具有良好的兔角膜保存效果,其理化性质稳定且安全,可保存兔角膜7~10 d。
[Abstract]:Objective to develop a kind of medium preservation solution of human serum cornea suitable for our country. Methods the cornea preservation solution of group A (10%) and group B (30%) was used to preserve rabbit cornea, and Optisol-GS corneal preservative was used as control group. The activity of corneal endothelial cells was detected by HE staining and ultrastructural observation by scanning electron microscope (SEM) on day 10 and 14 after preservation. Results there was no significant difference in corneal endothelial cell activity between group C and control group at the 14th day after corneal preservation. There was no significant difference in Optisol-GS between the two groups. The physicochemical properties of serum corneal preservation fluid were stable in each group. Compared with the control group, the rate of corneal endothelial cell activity in the non-biological hazard group was significantly different from that in the control group at the 3rd day after preservation (P 0.05), and the corneal histopathology was not significantly different between the experimental group and the control group on the 7th day after preservation, and there was no significant difference between the experimental group and the control group on the 7th day after preservation, there was no significant difference between the experimental group and the control group. The histopathological changes were observed successively in group B, and the ultrastructure was compared with each other. On the 7th day of preservation, there were significant differences between group D and group C and control group. Conclusion 30% human serum corneal preservation solution has a good effect on rabbit cornea preservation, its physical and chemical properties are stable and safe, and it can preserve rabbit cornea for 710 days.
【作者单位】：重庆医科大学附属第一医院眼科重庆市眼科学重点实验室;
【分类号】：R779.65

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