兔成肌细胞移植治疗失神经性声门关闭不全的实验研究

发布时间：2018-03-28 20:03

本文选题：成肌细胞　切入点：声门关闭不全　出处：《安徽医科大学》2010年硕士论文

【摘要】： 目的:观察兔喉返神经切断术后成肌细胞移植能否减缓甲杓肌肌萎缩及促进运动终板再生。方法:建立单侧失喉返神经动物模型20只兔被随机分为2组,均切断左侧喉返神经。成肌细胞培养无菌条件下取实验组胸锁乳突肌(约10g),采用酶消化法分离培养成肌细胞,一月后细胞扩增至1×106,个/ml时注射于失喉返神经的甲杓肌及声带内。对照组注射生理盐水。细胞的体外培养过程中,对其进行结蛋白及α-肌动蛋白的免疫组织化学鉴定。动物饲养2周及2月后处死动物,测量双侧甲杓肌湿重,行HE染色及乙酰胆碱酯酶组织化学染色进行观察。用Motic image advanced 3.2图像分析系统测定肌纤维横截面积。实验组和对照组均采用手术侧与自体健侧的测定值之比,得出失神经甲杓肌湿重的残存率、失神经甲杓肌横截面积的残余率。所得数据以均数±标准差表示,采用SPSS 11.5统计学软件分析,行两组样本均数t检验。结果:术后2周和8周,测量双侧甲杓肌湿重,得出甲杓肌湿重残存率,实验组分别为74.28%±8.39%及45.37%±6.78%,对照组分别为53.57%±2.52%及27.89%±3.14%,甲杓肌纤维横截面积残余率实验组分别为70.55%±7.12%和57.76%±6.27%,对照组分别为52.23%±4.76%和32.63%±3.41%;实验组均优于对照组,差异有统计学意义(P 0.05)。总结:成肌细胞移植可减轻失神经甲杓肌萎缩,可为是失神经性声门关闭不全提供治疗方法
[Abstract]:Aim: to observe whether the transplantation of myoblasts after recurrent laryngeal nerve transection can slow the atrophy of thyroarytenoid muscle and promote the regeneration of motor endplate. Methods: twenty rabbits with unilateral recurrent laryngeal nerve were randomly divided into two groups, and the left recurrent laryngeal nerve was cut off. The sternocleidomastoid muscle of the experimental group was isolated and cultured by enzyme digestion under the condition of sternocleidomastoid muscle culture. One month later, the cells were expanded to 1 脳 106, injected into the thyroid arytenoid muscle and vocal cord of the recurrent laryngeal nerve at a dose of 1 / ml, while the control group was injected with normal saline, and the cells were cultured in vitro. The desmin and 伪 -actin were identified by immunohistochemistry. The animals were killed for 2 weeks and 2 months later, and the wet weight of the bilateral thyroarytenoid muscle was measured. He staining and acetylcholinesterase histochemical staining were performed. The cross sectional area of muscle fiber was measured by Motic image advanced 3.2 image analysis system. The ratio of the operative side to the autologous normal side was used in the experimental and control groups. The residual rate of wet weight of denervated thyroarytenoid muscle and the residual rate of cross sectional area of denervated thyroarytenoid muscle were obtained. The data were expressed as mean 卤standard deviation and analyzed by SPSS 11.5 software, and the mean t test of two groups of samples was performed. Results: the wet weight of bilateral thyroarytenoid muscle was measured at 2 and 8 weeks after operation, and the residual rate of wet weight of thyroarytenoid muscle was obtained. In the experimental group, 74.28% 卤8.39% and 45.37% 卤6.78, 53.57% 卤2.52% and 27.89% 卤3.14 in the control group, 70.55% 卤7.12% and 57.76% 卤6.27% in the experimental group, 52.23% 卤4.76% and 32.63% 卤3.41% in the control group, respectively. Conclusion: myoblast transplantation can reduce the atrophy of denervated thyroarytenoid muscle and provide a therapeutic method for denervated glottic insufficiency.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R767

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