不同抗原修复方法对内翻性乳头状瘤石蜡切片Pan染色的影响

发布时间：2018-03-28 21:30

本文选题：鼻腔鼻窦内翻性乳头状瘤　切入点：免疫组织化学　出处：《临床耳鼻咽喉头颈外科杂志》2014年17期

【摘要】：目的:探讨不同修复条件及修复液对细胞角蛋白(广谱)单克隆抗体(Pan)的阳性表达程度的影响,筛选优势抗原修复方法。方法:对11例鼻腔鼻窦内翻性乳头状瘤(SNIP)组织石蜡切片,分别采用高温高压EDTA修复、微波EDTA修复、微波柠檬酸钠修复、高温高压柠檬酸钠修复,利用Image-Pro Plus图像分析仪对每张切片随机取5个视野,平均计算200个单位染色区域面积,测定染色区阳性反应的平均吸收度值。结果:高温高压柠檬酸钠修复、微波EDTA修复、微波柠檬酸钠修复与高温高压EDTA修复Pan的阳性表达分别为0.324±0.051、0.325±0.056、0.303±0.061及0.365±0.023;前3种方法与高温高压EDTA修复比较,均差异有统计学意义(均P0.05)。结论:应用高温高压EDTA修复液可最大化增加Pan的阳性表达强度,达到最佳染色结果,是一种值得推广的免疫组织化学修复方法,可为临床工作提供更好的指导作用。
[Abstract]:Objective: to investigate the effect of different repair conditions and solutions on the expression of cytokeratin (Broad) monoclonal antibody Pan. Methods: paraffin sections of 11 cases of inverted papilloma of nasal cavity and paranasal sinuses were treated with high temperature and high pressure EDTA repair, microwave EDTA repair, microwave sodium citrate repair, high temperature and high pressure sodium citrate repair, respectively. Image-Pro Plus image analyzer was used to randomly select 5 visual fields for each section. The average area of 200 units of staining area was calculated, and the average absorbance of positive reaction was measured. Results: high temperature and high pressure sodium citrate repair and microwave EDTA repair were used. The positive expression of Pan in microwave sodium citrate repair and high temperature and high pressure EDTA repair were 0.324 卤0.051 卤0.325 卤0.056 卤0.303 卤0.061 and 0.365 卤0.023 respectively. Conclusion: the application of high temperature and high pressure EDTA repair solution can maximize the positive expression intensity of Pan and achieve the best staining result. It is a kind of immunohistochemical repair method worth popularizing. It can provide better guidance for clinical work.
【作者单位】：大连医科大学附属第二医院耳鼻咽喉科;大连医科大学组胚实验室;
【基金】：国家自然科学基金项目(No:31371218)
【分类号】：R739.62

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