IL-25在慢性鼻-鼻窦炎伴鼻息肉患者血液及组织中的表达

发布时间：2018-03-29 15:03

本文选题：白细胞介素-　切入点：鼻窦炎　出处：《临床耳鼻咽喉头颈外科杂志》2014年20期

【摘要】：目的:观察IL-25在慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)患者血液及组织中的表达情况,探讨其在CRSwNP发生发展中的可能作用机制。方法:酶联免疫(ELISA法)分别检测CRSwNP组(68例)和鼻中隔偏曲组(对照组,55例)血液中IL-25的表达情况;免疫组织化学(LSAB法)分别检测CRSwNP组和对照组下鼻甲黏膜组织中IL-25的表达情况;全血细胞分析法测定两组患者血液中嗜酸粒细胞含量;苏木精-伊红染色法观察两组患者组织中嗜酸粒细胞浸润程度。结果:IL-25在CRSwNP组血液中含量及局部组织内阳性细胞计数显著高于对照组,差异有统计学意义(P0.01),且IL-25在CRSwNP组局部组织内表达与局部浸润的嗜酸粒细胞数呈正相关(r=0.679,P0.01)。结论:IL-25在CRSwNP患者体内表达增高,可能与嗜酸粒细胞浸润互为促进,在CRSwNP发生发展中具有重要的作用。
[Abstract]:Objective: to observe the expression of IL-25 in blood and tissues of patients with chronic rhinosinusitis and nasal polyps. Methods: the expression of IL-25 in the blood of 68 cases of CRSwNP group and 55 cases of nasal septum deviation group (control group 55 cases) was detected by enzyme-linked immunosorbent assay (Elisa). The expression of IL-25 in the mucosa of inferior turbinate in CRSwNP group and control group was detected by immunohistochemistry, and the eosinophil content in blood of two groups was determined by whole blood cell analysis. The infiltration of eosinophils in two groups was observed by hematoxylin-eosin staining. Results the blood content and the number of positive cells in local tissue of CRSwNP group were significantly higher than those in control group. The difference was statistically significant (P 0.01), and there was a positive correlation between the expression of IL-25 and the number of locally infiltrated eosinophils in the CRSwNP group. Conclusion the increase of the expression of IL-25 in CRSwNP patients may promote the infiltration of eosinophils. It plays an important role in the development of CRSwNP.
【作者单位】：首都医科大学附属北京朝阳医院耳鼻咽喉头颈外科;首都医科大学附属北京朝阳医院病理科;首都医科大学附属北京朝阳医院呼吸基础研究室;
【基金】：北京市教育委员会科技发展计划项目(No:KM201210025027)
【分类号】：R765.4

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