不同浓度甲泼尼龙琥珀酸钠鼓室内给药对其内耳受体表达的影响

发布时间：2018-03-29 20:46

本文选题：内耳　切入点：受体　出处：《安徽医科大学学报》2010年04期

【摘要】：目的探讨不同浓度的甲泼尼龙琥珀酸钠(MTH)经鼓室内给药后对豚鼠内耳中糖皮质激素受体表达的影响,分析类固醇激素鼓室内给药的可能机制,为临床治疗及用药提供理论依据。方法经鼓室注射32、40、62.5g/L3种不同浓度的MTH溶液,分别于注射后1、1.5、2h断头取耳蜗,用糖皮质激素单克隆抗体,采用免疫组化SP方法检测糖皮质激素受体(GR)在豚鼠耳蜗中的表达。结果不同浓度MTH鼓室内给药后糖皮质激素受体在豚鼠耳蜗各回均有不同程度的染色,主要阳性部位是螺旋韧带、血管纹、螺旋器和螺旋神经节。与正常组相比不同浓度的MTH鼓室内给药后糖皮质激素受体在耳蜗各阳性部位的表达均明显增强,且同一时间点浓度越高其受体表达越强,而同一浓度又以1.5h时间点表达最强,即在1.5h取材点、62.5g/LMTH鼓室内给药后糖皮质激素受体在豚鼠内耳中表达强度最大,其中在螺旋韧带和血管纹的表达增强最大,有统计学意义(P0.05)。3种浓度的MTH鼓室内给药后2h取材时间点的ABR波形Ⅲ波阈值较对照组差异均无统计学意义(P0.05)。结论 MTH鼓室内给药可使糖皮质激素受体在豚鼠耳蜗中的表达增强。且其表达强度和时间及浓度有关。
[Abstract]:Objective to investigate the effects of different concentrations of methylprednisolone sodium succinate (MTH) effect after intratympanic administration of glucocorticoid receptor expression in the inner ear of guinea pigs, intratympanic steroid hormone analysis the possible mechanism of drugs, and provide a theoretical basis for clinical treatment and medication. Methods intratympanic injection of 32,40,62.5g/L3 with different concentration MTH solution injection respectively after 1,1.5,2h removed the cochlea, using glucocorticoid monoclonal antibody by immunohistochemical SP method to detect the glucocorticoid receptor (GR) expression in the cochlea of guinea pigs. The results of different concentrations of MTH after intratympanic glucocorticoid receptor in the guinea pig cochlea each to have different degrees of dyeing, the main parts of the spiral ligament is positive, stria vascularis, spiral ganglion and organ. Compared with the normal group of different concentrations of MTH after intratympanic glucocorticoid receptors in different parts of the cochlea was positive significantly At the same time increased, and the higher the concentration of its receptor expression is stronger, and the same concentration in 1.5h time point was the strongest, which is based in 1.5h, 62.5g/LMTH after intratympanic administration of glucocorticoid receptor expression in the inner ear of guinea pig in the largest, which enhanced the maximum expression in stria vascularis and spiral ligament. There was statistical significance (P0.05).3 concentrations of MTH after intratympanic 2H ABR waveform III wave threshold at each time point compared with the control group showed no significant difference (P0.05). Conclusion MTH can make the intratympanic glucocorticoid receptor expression in guinea pig cochlea and its expression intensity and enhancement. The time and concentration.

【作者单位】：安徽医科大学附属省立医院耳鼻咽喉头颈外科;
【分类号】：R764

【共引文献】