微生态环境对小鼠变应性鼻炎发病率的影响

发布时间：2018-03-31 23:03

本文选题：鼻炎　切入点：变应性　出处：《临床耳鼻咽喉头颈外科杂志》2014年23期

【摘要】：目的:探讨微生态环境的改变对小鼠变应性鼻炎(AR)发病率的影响。方法:将60只新生的BALB/c小鼠随机分为2组,一组置于超净无菌环境(GF环境组),另一组置于无特定病原体环境(SPF环境组)中饲养,每组30只。2种环境中的小鼠再各分为模型组(20只)和对照组(10只)。2组环境组中的模型组各在6周龄时用卵清蛋白致敏并激发,观察其症状和体征并评分;于第9周末次激发实验后取材,用苏木精-伊红染色法行鼻腔组织切片染色;应用ELISA法检测外周血血清中IgE、IFN-γ和IL-4浓度。结果:各组小鼠症状体征评分显示,GF环境模型组中AR反应阳性体征的小鼠数量显著高于SPF环境模型组;苏木精-伊红染色显示AR反应阳性小鼠鼻黏膜高度充血水肿,有大量炎性细胞浸润,各模型组中AR反应阴性的小鼠和对照组的小鼠鼻黏膜无异常形态;嗜酸粒细胞计数显示AR反应阳性小鼠鼻黏膜内嗜酸粒细胞明显增多;ELISA结果显示AR反应阳性小鼠血清中IgE、IL-4浓度显著升高(P0.05),IFN-γ浓度显著降低(P0.05)。结论:GF环境中小鼠AR的发病率高于SPF环境。
[Abstract]:Objective: to investigate the effect of microecological changes on the incidence of allergic rhinitis in mice. Methods: sixty newborn BALB/c mice were randomly divided into two groups. One group was fed in a super sterile environment (GF group) and the other group was fed in a pathogen free environment (SPF group). 30 mice in each group were divided into two groups: model group (n = 20) and control group (n = 10). The model group was sensitized and stimulated with ovalbumin at 6 weeks of age. The symptoms, signs and scores were observed. At the end of the 9th week, the samples were collected and stained with hematoxylin and eosin staining. Results: the scores of symptoms and signs in each group showed that the number of mice with AR positive signs in SPF environment model group was significantly higher than that in SPF environment model group. Hematoxylin and eosin staining showed that the nasal mucosa of AR positive mice was hyperemia and edema with a large number of inflammatory cells infiltrating. Eosinophil count showed that the number of eosinophils in nasal mucosa of AR positive mice was significantly increased. Elisa results showed that the concentration of IgEN IL-4 in serum of AR positive mice was significantly increased, and the concentration of IFN- 纬 decreased significantly. Conclusion the hair of AR in mice in the environment of 0: GF is significantly decreased. The disease rate was higher than that in SPF environment.
【作者单位】：第四军医大学西京医院耳鼻咽喉头颈外科;第四军医大学西京医院骨科;
【基金】：国家自然科学基金资助项目(No:81470031)
【分类号】：R765.21

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