钙调素在正常人及不同年龄白内障患者晶状体上皮细胞中的表达

发布时间：2018-04-01 13:35

本文选题：钙调素　切入点：白内障　出处：《眼科新进展》2014年01期

【摘要】：目的检测钙调素(calmodulin,CaM)在正常人及不同年龄白内障患者晶状体上皮细胞(lens epithelial cells,LEC)中的表达情况,探讨CaM与白内障发生发展的关系及其表达在白内障患者中是否有年龄相关性。方法收集18~28岁角膜移植患者的正常晶状体前囊膜12例,1~18岁(儿童组)、18~45岁(青年组)、45~60岁(中年组)、60岁(老年组)白内障患者手术中环形撕除的晶状体前囊膜各12例,经固定、脱水、包埋、切片处理,间接免疫荧光方法检测CaM的表达量,拍照,IPP 6.0计算CaM的荧光值,SPSS 16.0进行统计学分析。结果 CaM在LEC中有表达,且主要表达在细胞浆,细胞核也有少量表达。同年龄组相比,青年组白内障患者LEC中CaM的表达量(0.507±0.288)高于正常组(0.297±0.007),差异有统计学意义(F=17.448,P=0.021);儿童组(0.624±0.207)、青年组(0.507±0.288)、中年组(0.704±0.168)、老年组(0.561±0.288)白内障患者间LEC中CaM的表达比较,差异均无统计学意义(均为P0.05)。结论 CaM在人LEC的表达与白内障的发生发展相关,可能是晶状体损伤白内障发生的一个标志,CaM在白内障LEC中表达无年龄相关性。
[Abstract]:Objective to detect the expression of calmodulin calmodulin Cam in lens epithelial cells of lens epithelial cells (LECs) in normal subjects and cataract patients of different ages. To investigate the relationship between CaM and cataract development and whether the expression of CaM is age-related in cataract patients. Methods 12 cases of normal lens anterior capsule in 18 ~ 28 years old patients with corneal transplantation were collected. In the middle age group (middle age group, 60 years old group), 12 cases of anterior capsular capsular of the lens were removed during cataract surgery. After immobilization, dehydration, embedding, section processing, indirect immunofluorescence method, the expression of CaM was detected, and the fluorescence value of CaM was calculated by IPP6.0 for statistical analysis. Results CaM was expressed in LEC, and mainly expressed in cytoplasm. There is also a small amount of expression in the nucleus. Compared with the age group, The expression of CaM in LEC of young cataract patients (0.507 卤0.288) was higher than that of normal group (0.297 卤0.007), the difference was statistically significant (P < 0.05), and the expression of CaM in LEC of children group (0.624 卤0.207), young group (0.507 卤0.288), middle-aged group (0.704 卤0.168), and senile group (0.561 卤0.288) were compared. Conclusion the expression of CaM in human LEC is related to the occurrence and development of cataract, and it may be a marker of lens injury cataract occurrence. There is no age correlation between the expression of Cam in cataract LEC and the expression of Cam in cataract LEC.
【作者单位】：广西医科大学第一附属医院眼科;
【基金】：国家自然科学基金资助(编号:81360146) 广西自然科学基金资助(编号:桂科攻1140003A29)~~
【分类号】：R776.1

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