IL-2与白内障超声乳化术后干眼的相关研究

发布时间：2018-04-03 04:01

本文选题：白内障超声乳化术　切入点：泪膜稳定性　出处：《中南大学》2011年硕士论文

【摘要】：目的：通过检测泪液中细胞因子白细胞介素-2(IL-2)在白内障超声乳化手术(Phaco)术后含量的变化规律,探讨IL-2与Phaco术后干眼的关系。方法：收集2010年1月至2010年6月期间于长治医学院附属和济医院行白内障超声乳化手术患者78例,所有患者均由同一人行白内障超声乳化术联合人工晶体植入术(Phaco+IOL),术后均常规给予妥布霉素／地塞米松(典必舒)滴眼液。根据干眼诊断标准,所有患者于术后2周时分为两组：干眼组；非干眼组。所有患者分别于术前、术后2周、4周依次行泪膜破裂时间(break-up time BUT)、泪液分泌试验(Schirmer test I)、角膜荧光素染色试验(Fluorescein staining of cornea, CFL)。三项检查术眼均测量3次,取平均值作为最终结果。全部检查均由同一医师在同一暗室内进行,暗室内保持安静无风、恒温恒湿的条件。分别于术前、术后2周、术后4周取患者泪液,用双抗体夹心酶联免疫吸附法(ELISA)检测泪液中IL-2浓度,采用SPSS13.0统计软件对数据进行统计分析。结果：干眼组在术后2周、4周时与术前比较,泪膜破裂时间缩短、基础泪液分泌量降低、角膜荧光染色积分增加,差异具有统计学意义(所有P0.01)。干眼组在术后4周时与术后2周相比,泪膜破裂时间增加、基础泪液分泌量升高、角膜荧光染色积分降低,差异具有统计学意义(所有P0.01)。非干眼组在术后2周时与术前比较,泪膜破裂时间、基础泪液分泌量、角膜荧光染色积分均恢复到术前水平,差异无统计学意义(所有P0.05)。两组间术后2周、4周比较,差异均有统计学意义(P0.01)。干眼组在术后2周、术后4周时与术前比较,泪液中IL-2含量升高,有显著差异,其差异具有统计学意义(P0.01)；术后2周与4周比较,差异具有统计学意义。非干眼组术后2周、4周时术前相比,泪液中IL-2含量恢复到术前水平,其差异无统计学意义(P0.05)。两组间术后2周、4周比较,差异均有统计学意义(P0.01)。结论： 1、Phaco术后早期部分患者出现干眼症状。 2、IL-2在干眼患者中表达增高,提示IL-2在干眼发病机制中起一定的作用。
[Abstract]:Aim: to investigate the relationship between IL-2 and dry eye after phacoemulsification by detecting the content of interleukin-2 (IL-2) in lacrimal fluid after phacoemulsification.Methods: a total of 78 patients underwent phacoemulsification from January 2010 to June 2010 in Heji Hospital of Changzhi Medical College.All the patients were treated with phaco IOL after cataract phacoemulsification and intraocular lens implantation and were given tobramycin / dexamethasone eye drops.According to the criteria of dry eye diagnosis, all patients were divided into two groups at 2 weeks after operation: dry eye group and non dry eye group.All the patients were treated with tear film break-up time UTT, tear secretion test with Schirmer test I, corneal fluorescein staining of cornea, and CFL with fluorescein staining before operation and 2 weeks and 4 weeks after operation respectively.All eyes were measured 3 times, and the mean value was taken as the final result.All examinations were performed by the same physician in the same dark room, which kept quiet and windless, constant temperature and humidity.Tear samples were collected from patients before, 2 weeks and 4 weeks after operation respectively. The concentration of IL-2 in tears was detected by double antibody sandwich enzyme-linked immunosorbent assay (Elisa). The data were statistically analyzed by SPSS13.0 software.Results:In the dry eye group, the time of lacrimal film rupture was shortened, the amount of basal tear secretion was decreased, and the fluorescent staining score of cornea was increased at 2 weeks and 4 weeks after operation. The difference was statistically significant (all P 0.01).In the dry eye group, the tear film rupture time increased, the basal tear secretion increased and the corneal fluorescent staining score decreased at 4 weeks after operation compared with 2 weeks after operation, the difference was statistically significant (all P 0.01).In the non-dry eye group, the time of tear film rupture, the amount of basal tear secretion and the score of corneal fluorescence staining all returned to the preoperative level at 2 weeks after operation, and there was no significant difference between the two groups (all P 0.05).There were significant differences between the two groups at 2 weeks and 4 weeks after operation (P 0.01).In the dry eye group, the content of IL-2 in tears increased significantly at 2 weeks and 4 weeks after operation, and the difference was statistically significant (P 0.01), and the difference was statistically significant 2 weeks after operation and 4 weeks after operation.In the non-dry eye group, the content of IL-2 in tears returned to the preoperative level at 2 weeks and 4 weeks after operation, but the difference was not statistically significant (P 0.05).There were significant differences between the two groups at 2 weeks and 4 weeks after operation (P 0.01).Conclusion:1 dry eye symptoms were found in some patients early after Phaco operation.2the increased expression of IL-2 in dry eye patients suggests that IL-2 may play a role in the pathogenesis of dry eye.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.66

【参考文献】