布地奈德对大鼠变应性鼻炎最轻持续炎症反应模型IL-12表达的影响

发布时间：2018-04-04 11:49

本文选题：鼻炎　切入点：变应性　出处：《临床耳鼻咽喉头颈外科杂志》2015年03期

【摘要】：目的:研究布地奈德对大鼠变应性鼻炎(AR)最轻持续炎症反应(MPI)模型的影响并观察IL-12的表达变化。方法:60只健康SD大鼠随机分为A、B、C、D共4组,每组15只。A组为阳性对照组即AR组,B组为实验组即MPI给药组,C组为阴性对照组即MPI不给药组,D组为空白对照组。首先以含卵清蛋白(OVA)和氢氧化铝凝胶的生理盐水混合液对A、B、C 3组行基础致敏和强化致敏,再分别用1%和0.01%的OVA溶液或生理盐水长期鼻腔激发,D组始终以生理盐水进行致敏和激发。第36天起每次激发前30min B组给予布地奈德喷鼻,其余各组给予生理盐水代替。观察豚鼠鼻腔激发后症状(喷嚏)变化并检测鼻黏膜中嗜酸粒细胞(EOS)浸润程度、上皮细胞内细胞间黏附分子1(ICAM-1)及IL-12的表达情况。结果:B组在以1%OVA溶液激发时,喷嚏平均次数明显多于D组(P0.05),而与A、C组相比差异均无统计学意义(P0.05);改用0.01%OVA溶液鼻腔激发并给药后,AR症状基本消失,与D组相比差异无统计学意义(P0.05),鼻黏膜内仍有少量EOS浸润,并可见上皮细胞ICAM-1轻度表达,但与D组相比差异无统计学意义(P0.05),B组IL-12阳性表达明显,与D组相比差异无统计学意义。结论:布地奈德可显著抑制AR模型迟发相炎症反应,提高大鼠AR MPI模型IL-12的表达。
[Abstract]:Aim: to study the effect of budesonide on the minimal persistent inflammatory response (MPI) and the expression of IL-12 in rats with allergic rhinitis.Methods Sixty healthy Sprague-Dawley rats were randomly divided into 4 groups: group A (n = 15) and group A (n = 15).At first, the basic sensitization and enhanced sensitization were performed in group A (OVA) containing ovalbumin (OVA) and sodium hydroxide gel.Group D was sensitized and stimulated by saline in 1% and 0.01% OVA solution or saline for a long time.After 36 days, 30min B group was given budesonide nasal spray and the other groups were given normal saline instead.The changes of nasal symptoms (sneezing) and the infiltration of eosinophils (EOS) in nasal mucosa, the expression of intercellular adhesion molecule 1 (ICAM-1) and IL-12 in epithelial cells were observed.Results the average number of sneezes in group B was significantly higher than that in group D when stimulated with 1%OVA solution, but there was no significant difference between group A and group C, and the symptoms of AR disappeared after nasal stimulation with 0.01%OVA solution.There was no significant difference between group D and group D (P 0.05). There was still a small amount of EOS infiltration in nasal mucosa and slight expression of ICAM-1 in epithelial cells. However, there was no significant difference between group D and group D in the expression of IL-12, but there was no significant difference between group D and group D in the positive expression of IL-12.Conclusion: budesonide can significantly inhibit delayed inflammatory response in AR model and increase the expression of IL-12 in AR MPI model.
【作者单位】：郑州大学第二附属医院耳鼻咽喉头颈外科;
【分类号】：R765.21

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