生长相关蛋白-43在听觉剥夺大鼠听皮层中的表达研究

发布时间：2018-04-08 19:25

本文选题：幼鼠　切入点：听觉剥夺　出处：《中华耳科学杂志》2014年04期

【摘要】：目的探索听觉系统发育和损伤后修复的神经可塑性的分子机制,探索GAP-43与幼鼠听皮层发育和可塑性的关系;方法应用免疫组织化学方法,检测正常幼鼠(3周龄、4周龄及8周龄大鼠)及耳毒性药物致聋鼠发育的不同阶段(2周2天龄大鼠氨基糖苷类抗生素致聋后5天、12天及40天,即致聋的3周龄、4周龄及8周龄大鼠)GAP-43在听皮层的阳性神经元表达变化;结果发现GAP-43在刚出生大鼠听皮层阳性神经元高表达,随发育表达逐渐降低,出生后3周(NC P3W)的大鼠听皮层平均每高倍视野阳性神经元数为111.50±4.90,出生后4周(NC P4W)为84.17±3.24,出生后8周(NC P8W)为66.67±4.17;耳蜗损伤后早期GAP-43在幼鼠听皮层的表达反应性升高;结论 GAP-43与幼鼠听皮层发育和可塑性密切相关,GAP-43可作为听皮层乃至听觉系统发育和可塑性的重要标志。
[Abstract]:Objective to explore the molecular mechanism of neural plasticity in the auditory system development and repair after injury, and to explore the GAP-43 in relation to cortical development and plasticity; method of immunohistochemical method was used to detect normal rats (3 weeks old, 4 week old and 8 week old rats) in different stages of development in mice and induced ototoxic deafness the drug (5 days, 2 week, 2 day old rat to aminoglycoside antibiotic induced deafness after 12 days and 40 days, namely hearing loss caused by 3 weeks of age, 4 and 8 week old rats) expression of GAP-43 positive neurons in the auditory cortex; the results showed that GAP-43 in newborn rats to high the expression of cortical neurons, decreased gradually with the development of expression, 3 weeks after birth (NC P3W) in the rat auditory cortex per HPF positive neurons was 111.50 + 4.90, 4 weeks after birth (NC P4W) 84.17 + 3.24, 8 weeks after birth (NC P8W) was 66.67 + 4.17; cochlea after the injury of early GAP-43 in the auditory cortex of rats Conclusion the expression of GAP-43 is closely related to the development and plasticity of auditory cortex in young rats. GAP-43 can be used as an important marker for the development and plasticity of auditory cortex and auditory system.

【作者单位】：中国人民解放军白求恩国际和平医院;中国人民解放军第260医院;
【分类号】：R764

【共引文献】