冰片对鼻咽癌细胞容积敏感性氯通道的激活作用

发布时间：2018-04-10 01:06

本文选题：冰片　切入点：氯通道　出处：《中国药理学通报》2014年12期

【摘要】：目的探讨冰片对低分化鼻咽癌(CNE-2Z)细胞氯通道的激活作用及对细胞容积的影响。方法采用全细胞膜片钳技术记录冰片激活CNE-2Z细胞膜电流,分析其电流特性;用活细胞图像法观察并测量冰片对细胞体积的影响。结果细胞外灌流冰片(20μmol·L-1)诱导CNE-2Z细胞膜氯通道开放,产生氯电流,该电流有明显外向优势,无电压及时间依赖性失活,其翻转电位接近氯离子平衡电位,该电流可被氯通道阻断剂tamoxifen抑制,细胞外灌流47%高渗溶液完全抑制该电流。细胞外灌流冰片30 min,细胞体积缩小约9.4%,该作用可被tamoxifen完全抑制。结论冰片可以激活低分化鼻咽癌细胞容积敏感性氯通道,导致细胞体积缩小,氯通道在冰片引起细胞体积缩小中起着重要作用。
[Abstract]:Objective to investigate the effect of borneol on the activation of chloride channels and cell volume in poorly differentiated nasopharyngeal carcinoma (NPC) cells.Methods whole cell patch-clamp technique was used to record the current of CNE-2Z membrane activated by borneol, and to analyze its current characteristics, and to observe and measure the effect of borneol on cell volume by living cell image method.Results extracellular borneol (20 渭 mol L ~ (-1)) induced the opening of chloride channel in CNE-2Z cell membrane and produced chlorine current. The current showed obvious extroversion, no voltage and time-dependent inactivation, and its reversal potential was close to the equilibrium potential of chloride ion.The current was inhibited by chloride channel blocker tamoxifen, and was completely inhibited by extracellular perfusion of 47% hypertonic solution.After 30 minutes of extracellular perfusion of borneol, the cell volume was reduced by about 9.4%, which could be completely inhibited by tamoxifen.Conclusion Borneol can activate the volume-sensitive chloride channels in poorly differentiated nasopharyngeal carcinoma cells, resulting in the reduction of cell volume. Chlorine channels play an important role in the reduction of cell volume induced by borneol.
【作者单位】：暨南大学医学院生理学系;暨南大学医学院药理学系;
【基金】：国家自然科学基金资助项目(No 81273539,81173064) 教育部基金项目(No 20124401110009) 广州市科技计划基金项目(No 2013J450015) 东莞科技计划基金项目(No2011108102006)
【分类号】：R739.63

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