突触内吞关键蛋白Dynamin在小鼠耳蜗内毛细胞中的表达研究

发布时间：2018-04-12 11:06

本文选题：耳蜗内毛细胞 + 内吞　；参考：《中华耳科学杂志》2014年04期

【摘要】：目的研究生理情况下Dynamin各亚型在小鼠耳蜗内毛细胞(IHC)的表达分布特点,为探讨其在毛细胞内可能发挥的生理功能以及与听信号传导的关系做铺垫。方法取成年小鼠耳蜗基底膜行免疫荧光染色,激光共聚焦显微镜观察Dynamin各亚型表达及定位分布情况。结果 Dynamin各亚型在小鼠耳蜗内毛细胞中均有表达。其中,Dyna-min-1表达于整个内毛细胞内,Dynamin-2点状分布在内毛细胞胞质,Dynamin-3高表达于内毛细胞核下近螺旋神经节区域。结论网格蛋白介导内吞过程是毛细胞内吞作用的重要机制,作为该过程中的关键蛋白,Dynamin各亚型在耳蜗内毛细胞中均有表达,提示Dynamin在内毛细胞中可能发挥着重要的生理功能。
[Abstract]:Objective to study the expression and distribution of Dynamin subtypes in mouse cochlea hair cells under physiological conditions, and to explore its physiological function and the relationship with auditory signal transduction.Methods Immunofluorescence staining was performed on the basal membrane of adult mouse cochlea. The expression and localization of Dynamin subtypes were observed by confocal laser microscopy.Results all subtypes of Dynamin were expressed in mouse cochlear hair cells.The expression of Dyna-min-1 in the cytoplasm of the inner hair cells was highly expressed in the proximal spiral ganglion region of the inner hair nucleus.Conclusion griddle protein mediated endocytosis is an important mechanism of endocytosis in hair cells. As a key protein in the process, dynamic in all subtypes is expressed in cochlear hair cells, suggesting that Dynamin may play an important physiological role in the inner hair cells.
【作者单位】：第三军医大学附属西南医院耳鼻咽喉-头颈外科;
【基金】：国家自然科学基金项目(30973301,81271080,81470694) 重庆市自然基金资助项目(NO:CSTC2009BB5170) 第三军医大学基金:2010XLC06
【分类号】：R764

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