去整合素Echistatin抑制糖尿病兔远期后发性白内障

发布时间：2018-04-15 11:00

本文选题：去整合素 + 后发性白内障　；参考：《眼科新进展》2014年06期

【摘要】：目的观察并探讨不同浓度去整合素(Echistatin,Ecs)对糖尿病兔远期后发性白内障(posterior capsular opacification,PCO)发生过程中晶状体上皮细胞(lens epithelial cells,LEC)增殖的抑制作用,以及其在眼内的安全性。方法建立糖尿病兔模型,随机分为对照组(A组,术毕前房注入蒸馏水0.2 mL);实验组:B组、C组、D组(术毕前房分别注入5.0×10-3μg·L-1、7.5×10-3μg·L-1、10.0×10-3μg·L-1Ecs),术后裂隙灯下观察角膜、前房及PCO情况。术后6周取材,常规石蜡切片,HE染色。用免疫组织化学法检测PCO发生过程中LEC增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达,TUNEL法检测角膜内皮细胞、视网膜神经节细胞层及内核层细胞的凋亡情况。结果 HE染色结果显示,术后6周各组角膜内皮细胞排列整齐、未见水肿,视网膜内界膜与各层组织结构完整,未见药物引起明显的组织损伤反应。各组间PCO分级差异有统计学意义(P=0.011)。免疫组织化学法检测各组后囊膜PCNA表达结果显示,A组、B组、C组、D组的平均光密度值分别为0.499±0.043、0.484±0.055、0.422±0.012、0.340±0.031,B组后囊膜上PCNA表达与A组差异无统计学意义(P0.05),余组间两两比较差异均有统计学意义(均为P0.05)。TUNEL法检测各组角膜内皮细胞、视网膜神经节细胞层及内核层细胞凋亡发现,各组间细胞凋亡比较均未见明显差异(均为P0.05)。结论 10.0×10-3μg·L-1Ecs对糖尿病兔远期PCO具有明显抑制作用,且对眼内相邻及重要组织无明显毒性作用,因此可选用此浓度进行后续研究。
[Abstract]:Objective to investigate the inhibitory effect of different concentrations of deintegrin Echistatin on the proliferation of lens epithelial cells during the development of posterior capsular opacifications in diabetic rabbits and its safety in the eyes.Methods Diabetic rabbit model was established and randomly divided into two groups: control group A, treated with distilled water 0.2 mL / L at the end of operation, and group C of experimental group B (5.0 脳 10-3 渭 g / L -1) and group D (5.0 脳 10-3 渭 g / L -1) respectively. The corneal, anterior chamber and PCO were observed by slit lamp.Samples were collected 6 weeks after operation, and HE staining was performed on routine paraffin sections.Immunohistochemical method was used to detect the expression of proliferating cell nuclear antigen-PCNA in PCO and Tunel method was used to detect the apoptosis of corneal endothelial cells, retinal ganglion cell layer and nuclear layer cells.Results the results of HE staining showed that the corneal endothelial cells were arranged neatly at 6 weeks after operation, no edema was found in the corneal endothelium, the inner boundary membrane of the retina and the tissue structure of each layer were intact, and no obvious tissue injury was induced by drugs.There was a significant difference in PCO grading among the groups (P = 0.011).There was statistical significance (P0.05).TUNEL method was used to detect corneal endothelial cells in each group).The apoptosis of retinal ganglion cell layer and nuclear layer showed no significant difference among the three groups (P 0.05).Conclusion 10.0 脳 10 ~ (-3) 渭 g L-1Ecs has obvious inhibitory effect on long-term PCO in diabetic rabbits, and has no obvious toxicity to adjacent and important tissues in the eyes, so this concentration can be used for further study.
【作者单位】：广西医科大学第一附属医院眼科;
【基金】：国家自然科学基金资助(编号:81160120)~~
【分类号】：R776.1

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