新型过氧化物酶体增殖物激活受体激动剂对角膜新生血管的抑制作用

发布时间：2018-04-18 16:01

  本文选题：过氧化物酶体增殖物激活受体γ + 激动剂　； 参考：《武汉大学学报(医学版)》2014年06期

【摘要】：目的:探讨过氧化物酶体增殖物激活受体γ(PPARγ)激动剂对角膜新生血管的抑制作用及机制。方法:SD大鼠随机分成A、B、C及D组,行角膜囊袋法制作角膜新生血管模型,右眼为实验眼。A组:空白对照组,正常角膜大鼠;B:阳性对照组,制作模型后生理盐水滴眼;C、D组:建模后分别滴用0.5%、1.0%PPARγ激动剂滴眼液。裂隙灯显微镜下测量新生血管面积,Western blot检测角膜血管内皮生长因子(VEGF)表达,ELISA检测房水VEGF的表达变化。结果:建模后第3,7,14,21,28天,角膜新生血管面积B组为(5.53±0.81)、(14.61±2.78)、(26.12±2.63)、(34.74±3.56)、(37.42±3.87)mm2,C组为(3.75±1.08)、(10.36±1.27)、(18.76±2.35)、(27.88±3.06)、(29.73±3.54)mm2,D组从第7天开始为(3.82±0.94)、(8.74±1.06)、(13.93±2.06)、(14.61±2.82)mm2。28dC、D组角膜新生血管抑制率分别为20.6%和61.0%,D组大鼠角膜新生血管面积小于B组和C组,差异有统计学意义(P0.05),而C组与B组之间差异无统计学意义(P0.05)。Western blot检测烧伤后14d新生血管角膜表达VEGF明显增强,C、D组VEGF相对量有不同程度下降。角膜损伤后,大鼠房水中VEGF表达随时间明显增多,高浓度的PPARγ激动剂滴眼液治疗后能明显降低房水VEGF的含量,第21天D组房水VEGF的表达明显低于B组[(687±126)pg/ml vs(1 260±104)pg/ml],差异具有统计学意义(P0.05)。结论:局部应用PPARγ激动剂能有效抑制角膜新生血管生成,抑制VEGF的合成和表达可能是阻止角膜新生血管生长机制的一部分。
[Abstract]:Aim: to investigate the inhibitory effect and mechanism of peroxisome proliferator activated receptor (PPAR 纬) agonist on corneal neovascularization.Methods Twenty SD rats were randomly divided into two groups: group C and group D, and the corneal neovascularization model was made with the method of corneal capsule. The right eye was divided into experimental eye (group A): blank control group (group A) and normal cornea group (group B: positive control group).After the establishment of the model, normal saline drops were used in group C: after modeling, 0.5 and 1.0% PPAR 纬 agonist eye drops were used respectively.Measurement of neovascularization area under slit lamp microscope Western blot was used to detect the expression of corneal vascular endothelial growth factor (VEGF). Elisa was used to detect the expression of VEGF in aqueous humor.缁撴灉:寤烘ā鍚庣��3,7,14,21,28澶，

本文编号：1769062