紫杉醇增强鼻咽癌细胞放射敏感性的初步机制研究

发布时间：2018-04-19 08:01

本文选题：鼻咽肿瘤 + 紫杉醇　；参考：《临床耳鼻咽喉头颈外科杂志》2014年15期

【摘要】：目的:评价紫杉醇对放射抗拒鼻咽癌细胞的放射增敏及其机制的初步研究。方法:通过X射线大剂量低分割照射技术建立鼻咽癌放射抗拒细胞亚系(子代,CNE-2S1);用紫杉醇单独处理和联合放疗分别处理CNE-2S1,以单纯放疗处理组为对照组。通过细胞克隆形成实验观察紫杉醇对放疗的增敏作用;采用流式细胞仪分析不同处理组CNE-2S1的细胞周期分布及凋亡发生率;免疫印迹分析不同处理组SHP-1表达水平。结果:成功建立鼻咽癌放射抗拒细胞亚系CNE-2S1;细胞克隆实验结果显示紫杉醇对放疗具有明显增敏作用;流式检查结果表明紫杉醇处理后的CNE-2S1处于G2-M期细胞比例明显增高,紫杉醇联合放疗处理组可明显提高CNE-2S1细胞的凋亡比率;免疫印迹结果显示紫杉醇及联合放疗组可降低CNE-2S1细胞中SHP-1的表达水平。结论:紫杉醇可增强放疗对鼻咽癌放射抗拒细胞的作用,SHP-1下调可能参与其中。
[Abstract]:Aim: to evaluate the radiosensitization and mechanism of paclitaxel on radiation-resistant nasopharyngeal carcinoma cells.Methods: nasopharyngeal carcinoma (NPC) resistant cell subline (CNE-2S1) was established by high dose and low fractionation X-ray irradiation. CNE-2S1 was treated with paclitaxel alone or combined with radiotherapy, and the control group was treated with radiotherapy alone.The sensitivity of paclitaxel to radiotherapy was observed by cell clone formation assay. The cell cycle distribution and apoptosis rate of CNE-2S1 in different treatment groups were analyzed by flow cytometry and SHP-1 expression levels in different treatment groups were analyzed by Western blot.Results: nasopharyngeal carcinoma cell line CNE-2S1 was successfully established, the results of cell cloning showed that paclitaxel significantly enhanced the sensitivity to radiotherapy, and flow cytometry showed that the proportion of CNE-2S1 in G2-M phase was significantly increased after paclitaxel treatment.The apoptosis rate of CNE-2S1 cells was significantly increased in paclitaxel combined with radiotherapy group, and the results of Western blot showed that paclitaxel combined with radiotherapy could decrease the expression of SHP-1 in CNE-2S1 cells.Conclusion: paclitaxel can enhance the effect of radiotherapy on nasopharyngeal carcinoma radioresistance cells. The down-regulation of SHP-1 may be involved.
【作者单位】：黄石市中心医院肿瘤内一科;华中科技大学同济医学院附属协和医院肿瘤中心;
【基金】：湖北省自然科学基金面上项目资助(No:2012FFB02324)
【分类号】：R739.63

【参考文献】