锌指转录调节因子KLF4蛋白在喉鳞状细胞癌组织中的表达及意义

发布时间：2018-04-19 13:47

本文选题：KLF4 + 喉鳞状细胞癌　；参考：《南华大学》2010年硕士论文

【摘要】： 目的观察锌指转录调节因子KLF4(Kruppel-like factor 4)蛋白在人喉鳞状细胞癌组织中及声带息肉组织中的表达的变化。初步分析KLF4在人喉鳞状细胞癌发病机制中的作用,为喉鳞状细胞癌的发病机制提供实验依据。方法1、免疫组织化学染色经病理确诊为喉鳞状细胞癌组织45例(声门型),观察KLF4的分布及表达情况,以48例声带息肉组织做对照(对照组)。根据阳性细胞数的比例和染色强度行半定量积分。2、用Western Bloting法检测人经病理确诊12例为喉鳞状细胞癌(声门型)组织中KLF4蛋白的含量,以及12例声带息肉组织中KLF4的含量。以β-actin为内对照。实验结果用Image-pro plus Version5.0.1图像分析系统进行灰度值测定。实验结果均采用SPSS13.0统计分析软件进行分析,p0.05为有统计学意义。结果1、免疫组化:喉鳞状细胞癌组织中KLF4的阳性表达率为48.9%(22/45)。在声带息肉组织中阳性表达率为89.6%(43/48)。两者间差异具有统计学意义,p 0.05。2、Western bloting:Image-pro plus Version5.0.1图像分析系统吸光度值,KLF4表达在喉鳞状细胞癌组织中较声带息肉中低,p 0.05,其表达差异有显著性。结论1锌指转录调节蛋白KLF4广泛表达在声带息肉上皮细胞中,主要分布在细胞质及细胞核膜,并且在声带息肉组织中的表达比较稳定;2、KLF4蛋白在声门型喉鳞状细胞癌中的表达低于声带息肉组织,两者间差异具有统计学意义。
[Abstract]:Objective to investigate the expression of zinc finger transcription regulator KLF4(Kruppel-like factor 4 in human laryngeal squamous cell carcinoma and vocal cord polyp.To analyze the role of KLF4 in the pathogenesis of human laryngeal squamous cell carcinoma and to provide experimental evidence for the pathogenesis of laryngeal squamous cell carcinoma.Methods 1. The distribution and expression of KLF4 in 45 cases of squamous cell carcinoma of larynx (glottic type) were observed by immunohistochemical staining, and 48 cases of vocal cord polyp were used as control group (control group).According to the proportion of positive cells and the intensity of staining, the content of KLF4 protein in 12 cases of laryngeal squamous cell carcinoma (glottic type) and the content of KLF4 in 12 cases of vocal cord polyp were detected by Western Bloting method.尾 -actin was used as internal control.The experimental results were measured by Image-pro plus Version5.0.1 image analysis system.The experimental results were analyzed by SPSS13.0 statistical analysis software.Results 1. Immunohistochemistry: the positive expression rate of KLF4 in laryngeal squamous cell carcinoma was 48.9% and 22 / 45%.The positive expression rate was 89.6% in vocal cord polyp tissue.The expression of KLF4 in laryngeal squamous cell carcinoma (LSCC) was significantly lower than that in vocal cord polyp (P 0.05), and there was a significant difference in the expression of KLF4 in laryngeal squamous cell carcinoma (LSCC) than in vocal cord polyp (P 0.05).Conclusion 1 zinc finger transcription regulatory protein (KLF4) is widely expressed in the epithelial cells of vocal cord polyps, mainly in the cytoplasm and nuclear membrane.The expression of KLF4 protein in glottic laryngeal squamous cell carcinoma was significantly lower than that in vocal polyp tissue.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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