IL-17和TNF-α对鼻息肉上皮细胞中MIP-3α含量的影响

发布时间：2018-04-20 21:22

本文选题：鼻息肉 + IL-17　；参考：《山西医科大学》2011年硕士论文

【摘要】：目的探讨前炎细胞因子白介素-17(IL-17)和肿瘤坏死因子-α(TNF-α)对鼻息肉上皮细胞中巨噬细胞炎性蛋白-3α(macrophage inflammatory protein -3α, MIP-3α/CCL20)的作用。方法离体培养人鼻息肉上皮细胞,不加刺激因子以及IL-17、TNF-α刺激细胞后,用酶联免疫吸附法(ELISA)测定上清液中MIP-3α/CCL20含量的变化,并用逆转录聚合酶链反应(RT-PCR)检测人鼻息肉上皮细胞中MIP-3α/CCL20 mRNA含量的变化。结果IL-17、TNF-α单独刺激鼻上皮细胞后,上清液中MIP-3α/CCL20的含量较不加刺激因子时增多,IL-17、TNF-α共同刺激下,上清液中MIP-3α/CCL20的含量较不加刺激因子、以及IL-17、TNF-α单独刺激时增加。并且IL-17、TNF-α的诱导作用具有剂量-时间依从性。同时测定鼻上皮细胞中MIP-3α/CCL20 mRNA的相对表达量,得到同样的结果。结论鼻息肉上皮细胞产生MIP-3α/CCL20,而且IL-17、TNF-α对其有诱导作用。MIP-3α/CCL20可能对T细胞和树突状细胞在鼻息肉的聚集中发挥重要的作用。
[Abstract]:Objective to investigate the effects of proinflammatory cytokines interleukin-17 (IL-17) and tumor necrosis factor- 伪 (TNF- 伪) on macrophage inflammatory protein -3 伪 macrophage inflammatory protein 3 伪 (MIP-3 伪 / CCL20) in nasal polyp epithelial cells. Methods Human nasal polyp epithelial cells were cultured in vitro. After stimulated by IL-17 TNF- 伪, the levels of MIP-3 伪 / CCL20 in the supernatant were measured by Elisa. The changes of MIP-3 伪 -CCL20 mRNA in human nasal polyp epithelial cells were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results after stimulation of nasal epithelial cells with IL-17 TNF- 伪 alone, the content of MIP-3 伪 / CCL20 in supernatant was higher than that without IL-17 TNF- 伪, and the content of MIP-3 伪 / CCL20 in supernatant was higher than that of non-stimulator and IL-17 TNF- 伪 alone. Moreover, the induction of IL-17 TNF- 伪 has dose-time compliance. At the same time, the relative expression of MIP-3 伪 / CCL20 mRNA in nasal epithelial cells was measured and the same results were obtained. Conclusion nasal polyp epithelial cells produce MIP-3 伪 / CCL20, and IL-17 TNF- 伪 induces it. MIP-3 伪 / CCL20 may play an important role in the aggregation of nasal polyps by T cells and dendritic cells.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R765.25

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