持续气道正压通气对阻塞性睡眠呼吸暂停低通气综合征患者外周血IL-7的影响

发布时间：2018-04-21 12:11

本文选题：睡眠呼吸暂停 + 阻塞性　；参考：《重庆医学》2014年30期

【摘要】：目的通过研究阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者经持续气道正压通气(CPAP)治疗前后外周血中IL-7和调节性T(Treg)细胞的水平变化,探讨IL-7和Treg细胞在OSAHS患者中病理生理机制。方法采集OSAHS患者(OSAHS组,51例)治疗前以及健康体检者(对照组,35例)人群外周静脉血标本,分别采用ELISA及流式细胞术(FCM)检测受试者外周血中IL-7和Treg细胞水平,并分析OSAHS患者外周血IL-7和Treg细胞水平与睡眠呼吸暂停低通气指数(AHI)和最低血氧饱和度(SaO2)的相关性;OSAHS组患者经CPAP治疗后再次检测上述各项指标,比较其治疗前后的水平变化。结果 OSAHS组患者治疗后外周血IL-7表达[(186.41±18.10)pg/mL]较治疗前明显增高[(87.39±5.16)pg/mL],Treg细胞数量在治疗后为(1.28±0.19)%,较治疗前[(0.95±0.11)%]明显升高。相关性分析显示,OSAHS患者外周血IL-7及Treg细胞水平与最低SaO2呈正相关(r=0.679,r=0.681,P0.01);OSAHS患者外周血IL-7及Treg细胞水平与AHI呈负相关(r=-0.653,r=-0.632,P0.01)。结论 OSAHS患者外周血中IL-7和Treg细胞水平较对照组降低可能是OSAHS患者高水平系统炎症的原因之一,CPAP治疗能有效提高OSAHS患者的IL-7与Treg细胞水平,改善患者机体免疫失衡状态。
[Abstract]:Objective to investigate the changes of IL-7 and TregT cells in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome (OSAHS) before and after continuous positive airway pressure ventilation (CPAP), and to explore the pathophysiological mechanism of IL-7 and Treg cells in OSAHS patients. Methods Peripheral venous blood samples were collected from 51 patients with OSAHS before treatment and 35 healthy controls (control group). The levels of IL-7 and Treg cells in peripheral blood were measured by ELISA and flow cytometry respectively. The correlation between the levels of IL-7 and Treg cells in peripheral blood of patients with OSAHS and the sleep apnea hypopnea index (AHIH) and the lowest oxygen saturation (SAO2) were analyzed. After CPAP treatment, the above indexes were detected again, and the changes of the above indexes were compared before and after treatment. Results after treatment, the expression of IL-7 in peripheral blood [186.41 卤18.10)pg/mL] in OSAHS group was significantly higher than that before treatment [87.39 卤5.16)pg/mL] after treatment, and 1.28 卤0.19% after treatment and 0.95 卤0.11% before treatment. Correlation analysis showed that the levels of IL-7 and Treg cells in peripheral blood of patients with OSAHS were positively correlated with the lowest SaO2. The levels of IL-7 and Treg cells in peripheral blood of patients with OSAHS were negatively correlated with AHI. Conclusion the lower levels of IL-7 and Treg cells in peripheral blood of patients with OSAHS may be one of the reasons for the high level of systemic inflammation in OSAHS patients. CPAP treatment can effectively improve the IL-7 and Treg cell levels in OSAHS patients and improve the immune imbalance of the patients.
【作者单位】：重庆医科大学附属第一医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金面上项目(81271061) 国家临床重点专科建设项目经费资助(卫办医政函[2012]649号)
【分类号】：R766

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