耳蜗毛细胞与血管纹的相互依存关系的研究

发布时间：2018-04-22 02:24

本文选题：Corti’s器 + 血管纹　；参考：《中华耳科学杂志》2014年03期

【摘要】：目的通过对Atoh1条件基因敲除小鼠及Mitf基因突变小鼠两个动物模型的研究,来验证毛细胞及血管纹两者的存活是否相互依赖,以及在内耳中是否存在钾离子从血管纹—毛细胞—支持细胞—血管纹循环通路。方法分别对一月龄两种模型小鼠进行ABR、CM、CAP和EP的测量。并用免疫组化和共聚焦显微镜以及耳蜗铺片、切片对两种模型小鼠的耳蜗Corti’s器和血管纹进行形态学观察。结果内淋巴的缺损可造成外毛细胞的凋亡,但内毛细胞依然能够存活。20 mV的内淋巴电位是外毛细胞存活所必须的。血管纹的生存及功能并不需要功能正常的Corti’s器的支持,同时内淋巴电位的产生及维持并不依赖毛细胞及支持细胞的钾离子循环的支持。结论成熟毛细胞的存活依赖于正常的血管纹功能和内淋巴的离子环境。然而,血管纹的存活及功能却并不依赖Corti’s器的功能。
[Abstract]:Objective to study two animal models of Atoh1 conditional gene knockout mice and Mitf gene mutation mice to verify whether the survival of hair cells and stria vascularis are interdependent. And whether potassium ion circulates from stria-hair cell-Sertoli cell-stria vascularis in the inner ear. Methods Abr CMN CAP and EP were measured in two model mice of one month old. The cochlea Corti's apparatus and stria vascularis were observed by immunohistochemistry confocal microscope and cochlea spreading slice. Results the endolymphatic defect could induce the apoptosis of the outer hair cells, but the endolymphatic potential of 20 MV was necessary for the survival of the outer hair cells. The survival and function of stria vascularis do not require the support of the functional Corti's apparatus, and the production and maintenance of endolymphatic potentials do not depend on the potassium cycling of hair cells and Sertoli cells. Conclusion the survival of mature hair cells depends on the normal stria function and the ion environment of endolymphes. However, the survival and function of stria vascularis do not depend on the function of Corti's.
【作者单位】：首都医科大学附属北京同仁医院耳鼻咽喉头颈外科;Department
【分类号】：R764

【共引文献】