鼻咽癌中8-OHdG及其相关分子的表达水平研究

发布时间：2018-04-22 13:50

本文选题：鼻咽癌 + 8-OHdG　；参考：《广西医科大学》2010年硕士论文

【摘要】： 目的:探讨8-OHdG及其相关分子hOGG1在鼻咽癌患者和正常人血清及尿液中的浓度水平变化及其意义,以及在鼻咽癌发生中的作用。方法:采用双抗体夹心酶联免疫吸附测定法( enzyme linked immunosorbentassay,ELISA)定量检测67例鼻咽癌患者及28例头颈部炎症患者、30例体检健康人血清和尿液中8-OHdG的水平,并用同法定量检测血清中hOGG1、EB-VCA-IgA、EB-EA-IgG的水平。结果:鼻咽癌血清8-OHdG的水平明显高于头颈部炎症组和健康体检组(P0.01);鼻咽癌尿8-OHdG的水平虽高于头颈部炎症组和健康体检组,但无统计学差异(P0.05);鼻咽癌血清和尿液中8-OHdG的水平与患者的性别、年龄无相关性(P㧐0.05);鼻咽癌血清中hOGG1含量明显低于头颈部炎症组和健康体检组(P0.05);鼻咽癌组血清中EB-VCA-IgA阳性率明显高于头颈部炎症组和健康体检组(X2=38.367,P=0.000);鼻咽癌血清中EB-EA-IgG阳性率明显高于头颈部炎症组和健康体检组(X2=26.768,P=0.000);鼻咽癌患者EB-VCA-IgA阳性/阴性组、EB-EA-IgG阳性/阴性组中血清和尿8-OHdG表达均无统计学差异(P0.05)。结论: 8-OHdG的表达水平升高及hOGG1的表达下调提示鼻咽癌患者可能存在更多的DNA氧化损伤,同时其修复能力一定程度上受到影响。鼻咽癌中EB-VCA-IgA、EB-EA-IgG与血清和尿8-OHdG水平无关,可能是与EBV检测方法及结果不能很好反映EBV在鼻咽粘膜中的潜伏感染有关。
[Abstract]:Objective: to investigate the changes and significance of 8-OHdG and its related molecule hOGG1 in serum and urine of patients with nasopharyngeal carcinoma (NPC) and normal controls, as well as its role in the pathogenesis of nasopharyngeal carcinoma (NPC). Methods: enzyme linked immunosorbent enzyme linked immunosorbent enzyme linked immunosorbent assay (Elisa) was used to detect the 8-OHdG levels in serum and urine of 67 patients with nasopharyngeal carcinoma and 28 patients with head and neck inflammation. The level of EB-VCA-IgAEA-IgG in serum was detected by the same method. Results: the level of serum 8-OHdG in nasopharyngeal carcinoma was significantly higher than that in head and neck inflammation group and healthy control group (P 0.01), although the level of urinary 8-OHdG in nasopharyngeal carcinoma was higher than that in head and neck inflammation group and healthy control group. However, there was no statistical difference (P 0.05). The levels of 8-OHdG in serum and urine of patients with nasopharyngeal carcinoma (NPC) were correlated with the sex of the patients. The serum hOGG1 level of nasopharyngeal carcinoma was significantly lower than that of head and neck inflammation group and healthy control group, the positive rate of serum EB-VCA-IgA in nasopharyngeal carcinoma group was significantly higher than that of head and neck inflammation group and healthy control group, and the positive rate of EB-EA-IgG in nasopharyngeal carcinoma serum was higher than that in head and neck inflammation group and healthy control group. The rate was significantly higher than that in the head and neck inflammation group and the healthy control group, and there was no significant difference in the expression of 8-OHdG in serum and urine in the patients with nasopharyngeal carcinoma (NPC) in EB-VCA-IgA positive and negative EB-EA-IgG positive / negative groups (P 0.05). Conclusion: the increased expression of 8-OHdG and the down-regulation of hOGG1 suggest that there may be more oxidative DNA damage in nasopharyngeal carcinoma patients, and its repair ability is affected to some extent. EB-VCA-IgACA EB-EA-IgG in nasopharyngeal carcinoma is not related to serum and urine 8-OHdG levels, but may be related to the detection of EBV in nasopharynx mucosa by EBV method and its results can not well reflect the latent infection of EBV in nasopharyngeal mucosa.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.63

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