TIMP-2基因转染豚鼠FDM模型后部巩膜MMP-2蛋白表达的变化

发布时间：2018-04-22 22:08

本文选题：形觉剥夺性近视 + 基质金属蛋白酶　；参考：《青岛大学》2011年硕士论文

【摘要】：目的观察外源性基质金属蛋白酶抑制剂-2(tissue inhibitor of matrixmetalloproteinase-2, TIMP-2)对形觉剥夺性近视(form-deprivation myopia, FDM)豚鼠后极部巩膜基质金属蛋白酶-2(matrix metalloproteinases-2, MMP-2)蛋白表达的影响。方法取3周龄健康豚鼠36只,采用眼罩遮盖法制备FDM豚鼠模型,随机分TIMP-2组、空质粒组、生理盐水组及对照组,每组9只,右眼为遮盖眼,TIMP-2组左眼为自身对照组。TIMP-2组、空质粒组、生理盐水组于形觉剥夺2周后分别注射等体积的脂质体介导的TIMP-2质粒,空质粒和生理盐水。于注药后的第2、7和14d处死豚鼠,取眼球后极部巩膜标本,用明胶酶普法检测MMP-2蛋白的表达。结果(1)各实验组间MMP-2蛋白表达：TIMP-2组后极部巩膜MMP-2酶原及活性酶表达降低,与自身对照组、对照组组间比较,转染第2、7、14d表达变化均有统计学意义(p0.05)。而空质粒组和生理盐水组与对照组相比,转染后第2、7、14d MMP-2蛋白表达差别无统计学意义(p0.05)。(2)TIMP-2组MMP-2蛋白的动态变化：TIMP-2组后极部巩膜MMP-2酶原及活性酶随转染时间不同而变化,转染后第2d开始降低,第7d达峰值,第14d略有增高,转染后第2d与第7、14d之间差别均有统计学意义(p0.05),第7d与第14d比较差异无统计学意义(p0.05)。而自身对照组没有明显变化,转染后第2、7、14之间差别没有统计学意义(p0.05)。结论外源性TIMP-2基因注入FDM豚鼠后,后极部巩膜MMP-2蛋白表达水平随转染时间延长出现先降低后略回升的动态改变,早期即可有效抑制后极部巩膜MMP-2蛋白的表达,减缓豚鼠FDM巩膜重塑的作用,但随着时间的延长,抑制作用有逐渐降低趋势。
[Abstract]:Objective to investigate the effect of exogenous matrix metalloproteinase-2 inhibitor of matrix metalloproteinase-2 (TIMP-2) on the expression of matrix metalloproteinase-2 (MMP-2) protein in posterior sclera of form-deprivation myopia (FDM) guinea pigs. Methods Thirty six 3-week-old healthy guinea pigs were randomly divided into TIMP-2 group, empty plasmid group, normal saline group and control group, with 9 in each group. The left eye of the right eye was covered with TIMP-2 group, and the left eye was the self-control group. TIMP-2 group was divided into three groups: the TIMP-2 group, the empty plasmid group, the normal saline group and the control group. The blank plasmid group and the normal saline group were injected with the same volume of liposome-mediated TIMP-2 plasmid, empty plasmid and normal saline respectively after 2 weeks of form deprivation. The guinea pigs were killed on the 2nd and 14th days after injection. The sclera specimens of the posterior pole of the eyeball were taken and the expression of MMP-2 protein was detected by gelatinase method. Results 1) the expression of MMP-2 protein in the posterior sclera of the control group and the control group was significantly lower than that of the control group and the control group. There was a significant difference between the two groups on the 14th day after transfection (p0.05). However, there was no significant difference in the expression of MMP-2 protein between the empty plasmid group and the saline group on the 2nd day after transfection. There was no significant difference in the expression of MMP-2 protein between the two groups. There was no significant difference in the expression of MMP-2 protein between the control group and the control group. The proto and active enzymes of MMP-2 in the polar sclera changed with the time of transfection. It began to decrease on the 2nd day after transfection, reached the peak on the 7th day, and increased slightly on the 14th day. There was significant difference between the 2nd day and the 7th day after transfection, but there was no significant difference between the 7th day and the 14th day. However, there was no significant change in the control group, and there was no significant difference between the two groups after transfection (P 0.05). Conclusion after exogenous TIMP-2 gene was injected into FDM guinea pigs, the expression of MMP-2 protein in the posterior pole sclera decreased first and then increased slightly with the extension of transfection time, and could effectively inhibit the expression of MMP-2 protein in the posterior pole sclera at the early stage. The effect of scleral remodeling in guinea pig FDM was slowed down, but the inhibitory effect decreased gradually with the prolongation of time.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R778.11

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