慢性间歇低氧大鼠中性多核白细胞与内皮细胞间接共培养炎症因子水平变化及抗氧化剂的干预作用

发布时间：2018-04-23 19:13

本文选题：间歇低氧 + 中性多核白细胞　；参考：《中国现代医学杂志》2014年02期

【摘要】：目的研究慢性间歇低氧(CIH)大鼠中性多核白细胞(PMNs)与内皮细胞间接共培养C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)水平的变化,探讨炎症因子水平的改变对内皮细胞的影响及抗氧化剂4-羟基-2,2,6,6-四甲基哌啶(Tempol)的干预作用。方法 36只雄性Wistar大鼠采用随机数字表法分为常氧对照组(NC组)、间歇低氧组(IH组)、间歇低氧Tempol干预组(IHT组)和间歇低氧盐水干预组(IHN组),每组9只。除NC组外均给予间歇低氧环境,IHT组低氧暴露前给予100 mg/(kg·d)的Tempol腹腔注射,生理盐水稀释,IHN组同时给予等体积生理盐水腹腔注射。暴露6周后处死大鼠,分离纯化PMNs。PMNs与正常大鼠主动脉内皮细胞Transwell间接共培养4 h,酶联免疫吸附法(ELISA)测定间接共培养上、下室上清中CRP、TNF-α和ICAM-1的水平。结果 NC组、IH组、IHT组和IHN组上、下室CRP、TNF-α和ICAM-1水平比较,差异均无统计学意义(均P0.05);与NC组下室相比,IH组和IHN组下室CRP、TNF-α和ICAM-1水平均明显升高,差异有统计学意义(P0.05);与IH组和IHN组下室比较,IHT组下室CRP、TNF-α和ICAM-1水平均明显降低,差异有统计学意义(均P0.05);IHT组下室与NC组下室相比,CRP、TNF-α和ICAM-1水平差异均无统计学意义(均P0.05);IH组下室与IHN组下室比较,以上指标差异均无统计学意义(均P0.05)。结论 CIH大鼠体内的PMNs与内皮细胞间接共培养,可上调CRP、TNF-α和ICAM-1等炎症因子水平,可能在CIH引起内皮损伤中发挥重要作用;抗氧化剂Tempol可能阻断炎症反应,干预CIH引起的内皮功能障碍的发生。
[Abstract]:Objective to study the changes of the levels of C-reactive protein (CRP), tumor necrosis factor- 伪 (TNF- 伪) and intercellular adhesion molecule-1 (ICAM-1) in chronic intermittent hypoxia (CIH) rat neutrophil polymorphonuclear leukocytes (PMNs) and endothelial cells (ECs). To investigate the effect of the change of inflammatory factor level on endothelial cells and the intervention effect of antioxidant 4-hydroxyl-2-dimethylpiperidine-6-tetramethylpiperidine (TMP) on endothelial cells. Methods Thirty-six male Wistar rats were randomly divided into control group (n = 9), intermittent hypoxia group (n = 9), intermittent hypoxia group (n = 9) and intermittent hypoxic Tempol group (n = 9). All the patients were given intraperitoneal injection of Tempol (100 mg/(kg d before hypoxic exposure) except NC group, and the same volume of saline was injected intraperitoneally in the saline dilution group (IHN group). After 6 weeks of exposure, the rats were killed, PMNs.PMNs was isolated and cultured with Transwell of normal rat aortic endothelial cells for 4 h, and the levels of CRPN-TNF- 伪 and ICAM-1 in the supernatants of indirect co-culture and inferior chamber were determined by enzyme-linked immunosorbent assay (Elisa). Results there was no significant difference in the levels of CRPNF-a and ICAM-1 between IHN group and IHT group in NC group (P 0.05), and the levels of CRP TNF- 伪 and ICAM-1 in IHN group and HIH group were significantly higher than those in NC group (P 0.05), and the levels of TNF- 伪 and ICAM-1 in IHN group were significantly higher than those in control group (P < 0.05). Compared with IH group and IHN group, the levels of TNF- 伪 and ICAM-1 in inferior ventricle of IHT group were significantly lower than those of IH group and IHN group. There was no significant difference in the levels of TNF- 伪 and ICAM-1 between the two groups (P 0.05). There was no significant difference in the above indexes between the two groups (P 0.05). Conclusion indirect co-culture of PMNs and endothelial cells in CIH rats may up-regulate the levels of inflammatory cytokines such as CRP- TNF- 伪 and ICAM-1, which may play an important role in endothelial injury induced by CIH, and the antioxidant Tempol may block the inflammatory response. Intervention in the occurrence of endothelial dysfunction caused by CIH.
【作者单位】：天津医科大学总医院呼吸科;
【基金】：国家自然科学基金(No:81170071)
【分类号】：R766

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