低能量激光照射对大鼠视神经不完全损伤作用的实验研究

发布时间：2018-04-25 16:59

本文选题：视神经 + 闪光视觉诱发电位　；参考：《中国医科大学》2010年硕士论文

【摘要】： 目的观察低能量激光照射对大鼠视神经损伤后伤侧眼闪光视觉诱发电位(F-VEP)潜伏时、视网膜神经节细胞(retinal ganglion cells,RGCs)数量、睫状神经营养因子(ciliary neurotrophic factor,CNTF)表达水平的影响。探讨低能量激光照射对大鼠视神经不完全损伤的影响及作用机制。方法随机抽取5只大鼠作为正常组,剩余的45只大鼠分为：伤后3d、7d、14d 3个时间点,每个时间点各15只大鼠再随机分为治疗组10只、对照组5只。除正常组外,全部大鼠均手术,造成视神经不完全损伤模型后,治疗组给予低能量激光照射治疗,对照组不给于处理因素。在损伤后1d、3d、5d、7d、10d、14d动态观测并比较对照组、治疗组大鼠伤侧眼F-VEP潜伏时变化。在相应的时间分别处死各时间点15只大鼠,取伤侧眼球固定、包埋,通过HE及免疫组化染色方法,测定对照组、治疗组及正常组RGCs数量、CNTF表达水平,并进行统计分析。结果视神经损伤后F-VEP潜伏时较正常眼明显延长。治疗组与对照组相比,损伤后3d、5d、7d和10d,潜伏时缩短,两组差异具有统计学意义(P0.05),而伤后1d和14d,F-VEP潜伏时差异无统计学意义(P0.05)。视神经不完全损伤后视网膜节细胞(RGCs)存活数量逐渐减少,损伤3天内减少速度慢,细胞数减少不明显,7天后RGCs减少速度加快,细胞数大量减少。在损伤后7d,治疗组RGCs存活数量较对照组增多,两组差异具有统计学意义(P0.05),而损伤后3d和14d,两组RGCs数差异无统计学意义(P0.05)。视神经不全损伤后,各时间点(3d、7d、14d)对照组CNTF表达IOD值均较正常组增加,两组各时间点差异均具有统计学意义(p0.05)。治疗组与对照组相比,在损伤后7天,两组视网膜CNTF表达的差异具有统计学意义(P0.05),而损伤后3天和14天,两组视网膜CNTF表达的差异无统计学意义(P0.05)。结论低能量激光照射治疗可缩短视神经不全损伤所致F-VEP潜伏时延长,缓解视神经损伤造成的视觉传导障碍；可增加视神经不全损伤后1周视网膜节细胞存活数量,减轻RGCs丢失的程度；可明显增加损伤后1周大鼠视网膜CNTF蛋白表达,提高视神经再生修复因子CNTF表达的数量。
[Abstract]:Purpose To observe the effects of low energy laser irradiation on the number of retinal ganglion cells and the expression of ciliary neurotrophic factor (CNTF) in the injured eyes of rats with optic nerve injury. To investigate the effect and mechanism of low energy laser irradiation on incomplete optic nerve injury in rats. Method Five rats were randomly selected as normal group. The remaining 45 rats were divided into 3 time points: 3 days after injury, 15 rats at each time point were randomly divided into treatment group (n = 10) and control group (n = 5). All the rats were operated on except the normal group. After the model of incomplete optic nerve injury was established, the treatment group was treated with low energy laser irradiation, while the control group was not given the factors of treatment. The dynamic changes of F-VEP in the injured eyes of rats in the treatment group were observed and compared with those in the control group at 1 day, 3 days, 5 days, 7 days, 10 days and 14 days after injury, and the changes of latent time of F-VEP in the injured eyes of the treatment group were compared. At the corresponding time, 15 rats were killed at each time point, and the injured side eyeball was fixed and embedded. The expression of RGCs in control group, treatment group and normal group was determined by HE and immunohistochemical staining, and the expression of RGCs was analyzed statistically. Result F-VEP latency after optic nerve injury was significantly longer than that in normal eyes. Compared with the control group, the latency time of the treatment group was shorter than that of the control group at 3 and 10 days after injury. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference between the two groups in the F-VEP latency at 1 and 14 days after injury (P 0.05). The survival rate of retinal ganglion cells (RGCs) decreased gradually after incomplete optic nerve injury, and the reduction rate of RGCs was slow in 3 days after injury. After 7 days, the decrease rate of RGCs increased and the number of cells decreased significantly. On the 7th day after injury, the number of RGCs survival in the treatment group was higher than that in the control group. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference in the RGCs number between the two groups on the 3rd day and the 14th day after injury (P 0.05). The IOD expression of CNTF in the control group was significantly higher than that in the control group, and the difference between the two groups at each time point was statistically significant (P 0.05). Compared with the control group, the expression of CNTF in the retina of the treatment group was significantly different from that in the control group on the 7th day after injury, but there was no significant difference in the expression of CNTF between the two groups on the 3rd and 14th day after injury. Conclusion Low energy laser irradiation can shorten the latency of F-VEP induced by incomplete optic nerve injury, alleviate the visual conduction disorder caused by optic nerve injury, and increase the number of retinal ganglion cells survival after 1 week of optic nerve incomplete injury. Reducing the loss of RGCs significantly increased the expression of CNTF protein in the retina and increased the expression of CNTF in the optic nerve regeneration repair factor 1 week after injury.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R454.2;R779.1

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