共同性外斜视内直肌Myf-5表达研究

发布时间：2018-04-28 00:26

本文选题：共同性外斜视 + 眼外肌　；参考：《青岛大学》2011年硕士论文

【摘要】：目的观察共同性外斜视患者内直肌中Myf-5的表达变化,探讨其在共同性外斜视发病机制中的作用。方法收集青岛大学医学院附属医院2009年12月-2010年11月住院手术治疗的共同性外斜视病人获得的内直肌段为斜视组,共16例。斜视组又根据年龄分为儿童斜视组(8例)和成人斜视组(8例)。另选同期行眼球摘除或角膜移植供体取得的内直肌为成人对照组(共6例)。所有的内直肌段固定切片后行Myf-5免疫组织化学染色,测Myf-5在眼外肌中表达的平均光密度值。对各组间平均光密度值进行比较,并对斜视患者眼外肌中Myf-5平均光密度值与斜视度及年龄进行Pearson相关性分析。结果①Mv.f-5的平均光密度值在斜视组0.024±0.041,成人对照组0.233±0.024,两者差异具有统计学意义(P0.01)：在儿童斜视组0.156±0.011,成人斜视组0.095±0.038,两者差异具有统计学意义(P0.01);成人对照组0.233±0.024,成人斜视组0.095±0.038,两者差异具有统计学意义(P0.01)。②斜视患者Myf-5表达与患者的斜视度和年龄呈负相关(r=-0.728,P=0.001,r=-0.643,P=0.004)。结论眼外肌中Myf-5表达的降低可能与斜视的发病有关,眼外肌中.Myf-5的表达变化可能与斜视的发展有关。
[Abstract]:Objective to investigate the expression of Myf-5 in rectus muscle of concomitant exotropia and its role in the pathogenesis of concomitant exotropia. Methods 16 patients with concomitant exotropia received from the affiliated Hospital of Qingdao University Medical College from December 2009 to November 2010 were selected as strabismus group. The strabismus group was divided into children strabismus group (n = 8) and adult strabismus group (n = 8). In addition, the medial rectus muscle obtained by eyeball extirpation or corneal transplantation was selected as the adult control group (6 cases). All the medial rectus segments were fixed with Myf-5 immunohistochemical staining, and the average optical density of Myf-5 expression in the extraocular muscle was measured. The mean optical density of the patients with strabismus was compared and the correlation between the mean optical density of Myf-5 and the degree of strabismus and age was analyzed by Pearson. Results the average optical density of 1Mv.f-5 was 0.024 卤0.041 in strabismus group and 0.233 卤0.024 in adult control group, the difference was statistically significant (P 0.01): 0.156 卤0.011 in children strabismus group, 0.095 卤0.038 in adult strabismus group, 0.233 卤0.024 in adult strabismus group, and 0.233 卤0.024 in adult strabismus group. The expression of Myf-5 in strabismus patients was negatively correlated with their strabismus degree and age. There was a negative correlation between the expression of Myf-5 and the patients' strabismus degree and age. Conclusion the decrease of Myf-5 expression in extraocular muscles may be related to the pathogenesis of strabismus, and the change of .Myf-5 expression in extraocular muscles may be related to the development of strabismus.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.6

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