喉咽鳞癌中CK4的表达及临床意义

发布时间：2018-04-28 07:31

  本文选题：细胞角蛋白4 + 喉咽肿瘤　； 参考：《青岛大学》2013年硕士论文

【摘要】：目的：探讨喉咽鳞癌组织中细胞角蛋白4(CK4)的表达及其与肿瘤发生、侵袭和转移的关系。 方法：应用免疫组织化学方法检测60例喉咽鳞癌患者的癌旁正常组织和癌组织标本中CK4的表达,应用VIDAS图像分析仪分析其图像的积分光密度(iOD),应用SPSS17.0进行统计学处理。 结果：在癌旁正常组织中,CK4表达明显、完整,而在鳞癌组织中,CK4的表达明显下降,其表达差异有显著性χ2=73.594, P0.05)。在喉咽鳞癌组织中CK4的表达随病理分化程度下降而下降,完整性亦逐渐破坏甚至消失,其变化的差异有统计学意义(P0.05)；CK4在T1-T2期的表达最强,在T3、T4期的表达依次减弱,其变化的差异有统计学意义(F=105.901,P0.05)；CK4在Ⅰ-Ⅱ期的表达最强,在Ⅲ期、Ⅳ期中的表达逐渐减弱,其变化的差异有统计学意义(F=138.815,P0.05)；CK4在无淋巴结转移的鳞癌组织中的表达较强,而在有淋巴结转移的鳞癌组织中表达较弱,其变化的差异有统计学意义(T=11.152,P0.05)；CK4表达阳性组较阴性组的综合治疗后5年生存率明显提高,P0.05。 结论：伴随着喉咽鳞癌病理学分化程度的降低CK4的表达逐渐降低；CK4在喉咽鳞癌组织中的低表达与肿瘤发生、侵袭和转移等生物学特性密切相关；CK4在喉咽鳞癌组织中的低表达对探讨喉咽鳞癌的生物学行为有一定的意义,并有望成为判断喉咽鳞癌恶性程度的生物学标记物。
[Abstract]:Objective: to investigate the expression of cytokeratin 4 CK4 in laryngeal squamous cell carcinoma (LSCC) and its relationship with carcinogenesis, invasion and metastasis. Methods: immunohistochemical method was used to detect the expression of CK4 in the normal tissues and tissues of 60 patients with larynx squamous cell carcinoma. The integral optical density (IOD) was analyzed by VIDAS image analyzer and SPSS17.0 was used for statistical analysis. Results: the expression of CK-4 in adjacent normal tissues was obvious and intact, while in squamous cell carcinomas, the expression of CK-4 was significantly decreased (蠂 ~ 2 / 73.594, P 0.05). In larynx squamous cell carcinoma, the expression of CK4 decreased with the decrease of pathological differentiation, and the integrality was gradually destroyed or even disappeared. The difference of the expression of CK4 in the T1-T2 phase was statistically significant, and the expression in T3 / T4 phase was decreased in turn. The expression of CK-4 in stage 鈪，

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