低频强噪声对大鼠听力的影响及内耳Caspase-3的表达

发布时间：2018-05-01 23:37

本文选题：低频噪声 + 耳蜗　；参考：《中华耳科学杂志》2014年03期

【摘要】：目的观察高强度低频噪声对听力及内耳细胞凋亡的影响。方法 52只大鼠随机分为正常对照组(Pr)13只和暴露组39只,暴露组包括即刻组(E0组)、3天组(E3组)和7天组(E7组)各13只,经强度为150dB中心频率在500Hz的稳态噪声持续暴露10分钟,分别于即刻、震后3天、7天,作脑干诱发电位检测,检测后处死动物,取材制作耳蜗石蜡切片行免疫组化观察。结果经150dB强噪声暴露后即刻,大鼠ABR阈值显著升高,3天组轻度恢复,7天组未进一步改善。暴露后各时间点ABR阈值较暴露前明显增高(P0.01),E0组与E3组、E7组之间有统计学差异(P0.05),E3组与E7组之间无统计学意义(P0.05)。免疫组化结果低频强声暴露后各组耳蜗Corti器、螺旋神经节细胞、血管纹和螺旋韧带均可见Caspase-3染色程度不同的阳性表达,对照组无明显表达,实验暴露组各时间点平均光密度值与对照组之间有统计学差异(P0.01),E0组与E3组、E7组之间有统计学差异(P0.05),E3组与E7组之间有统计学差异(P0.05)。结论高强度的低频噪声能够造成听力明显损伤,但可部分恢复,Caspase-3介导的内耳细胞凋亡可能在噪声性耳聋的发病机制中起重要作用。
[Abstract]:Objective to observe the effect of high intensity low frequency noise on hearing and apoptosis of inner ear cells. Methods Fifty-two rats were randomly divided into normal control group (n = 13) and exposure group (n = 39). There were 13 rats in the immediate group (E0 group) and 13 rats in the 7-day group (group E7). The intensity of 150dB center frequency was exposed to steady-state noise of 500Hz for 10 minutes. The brainstem evoked potentials were detected immediately and 3 days after the earthquake. The animals were killed and the paraffin sections of the cochlea were made for immunohistochemical observation. Results immediately after 150dB strong noise exposure, the ABR threshold was significantly increased in the group of 3 days with mild recovery and no further improvement was found in the group of 7 days. The ABR threshold at each time point after exposure was significantly higher than that before exposure. There was a significant difference between P0.01G E0 and E3 E7 groups. There was no significant difference between P0.05C E3 group and E7 group (P 0.05). Immunohistochemical results showed that positive expression of Caspase-3 was observed in cochlear Corti organ, spiral ganglion cells, stria vascularis and helical ligament in each group after low frequency strong sound exposure, but no significant expression was found in the control group. There was statistical difference between the average optical density at each time point in the experimental exposure group and the control group. There was a statistical difference between the E0 group and the E3 group and the E7 group. There was a statistical difference between the E3 group and the E7 group. Conclusion High intensity low frequency noise can cause hearing loss, but it may play an important role in the pathogenesis of noise-induced deafness by partially restoring Caspase-3 mediated apoptosis of inner ear cells.
【作者单位】：安徽医科大学解放军三零六临床学院耳鼻咽喉头颈外科;解放军第306医院耳鼻咽喉头颈外科;解放军第306医院病理科;国防科技大学光电科学与工程学院;
【分类号】：R764.433

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