鼻咽癌中HIF-2α、GLUT-1及OPN的表达及其意义

发布时间：2018-05-02 12:05

本文选题：鼻咽癌 + 乏氧诱导因子2α(HIF-2α)　；参考：《暨南大学》2010年硕士论文

【摘要】： 目的研究乏氧诱导因子2α(HIF-2a)、葡萄糖转运蛋白1(GLUT-1)及骨桥蛋白(OPN)蛋白在鼻咽癌中的表达及其与鼻咽癌生物学行为及放疗反应性之间的关系,探讨HIF-2a、GLUT-1及OPN在鼻咽癌诊断、临床分期、治疗及预后评估等方面的作用。方法应用免疫组织化学方法检测51例初诊鼻咽癌组织和20例鼻咽炎性组织中HIF-2a、GLUT-1及OPN蛋白的表达,并对29例患者放疗前、放疗期间、放疗结束时活检组织中HIF-2α、GLUT-1及OPN的蛋白表达进行动态检测。应用酶联免疫吸附试验(ELISA)检测30例鼻咽癌患者及20例正常人的血清OPN水平。结果鼻咽癌组织中HIF-2α、GLUT-1及OPN蛋白的阳性表达率分别为62.7%、49%和52.9%,明显高于鼻咽慢性炎性组织的20%、10%和10%(P0.05)。HIF-2a蛋白的表达与T分期、是否伴颈部淋巴结转移及临床分期相关(P0.05)。GLUT-1蛋白的表达与临床分期、是否伴有颈部淋巴结转移显著相关(P0.05),而两者的表达与患者性别、年龄无关(P0.05)。OPN蛋白的表达与患者的性别、年龄、T分期、是否伴颈部淋巴结转移及临床分期均无明显相关性(P0.05)。鼻咽癌组织中HIF-2α和GLUT-1蛋白的表达呈正相关(P0.01),OPN蛋白表达与HIF-2a和GLUT-1蛋白表达间未见相关性。29例动态观察的患者中,放疗期间HIF-2α、GLUT-1及OPN蛋白的表达强度较放疗前明显降低(P0.05)。鼻咽癌组血清OPN水平161.02±44.80ng/ml明显高于正常对照组58.89±24.65ng/ml,两者的差异具有统计学意义(P0.05)。血清OPN水平与临床分期、是否伴有颈部淋巴结转移相关(P0.05),而与患者性别、年龄、T分期无明显相关(P0.05)。鼻咽癌血清OPN水平与其肿瘤组织中HIF-2α、GLUT-1和OPN蛋白的表达之间未见相关性。结论HIF-2α、GLUT-1和OPN蛋白的高表达在鼻咽癌的发生、发展过程中有重要作用。共同检测HIF-2a与GLUT-1的表达对于鼻咽癌的临床分期、判断疗效及预后评估有重要意义。血清OPN水平检测可作为鼻咽癌的诊断、分期及预后判断的辅助指标。
[Abstract]:Objective to study the expression of hypoxia inducible factor 2 伪 (HIF-2a), glucose transporter (GLUT-1) and osteopontin (OPN) protein in nasopharyngeal carcinoma (NPC) and its relationship with the biological behavior and radiotherapy reactivity of nasopharyngeal carcinoma (NPC), and to explore the role of HIF-2aGLUT-1 and OPN in the diagnosis and clinical staging of nasopharyngeal carcinoma (NPC). The role of treatment and prognosis evaluation. Methods Immunohistochemical method was used to detect the expression of HIF-2aA1GLUT-1 and OPN protein in 51 nasopharyngeal carcinoma tissues and 20 nasopharyngitis tissues. The protein expression of HIF-2 伪 -GLUT-1 and OPN in biopsy tissue was detected dynamically at the end of radiotherapy. The serum OPN levels of 30 patients with nasopharyngeal carcinoma and 20 normal controls were detected by Elisa. Results the positive expression rates of HIF-2 伪 -GLUT-1 and OPN protein in nasopharyngeal carcinoma were 62.7% and 52.9%, respectively, which were significantly higher than those in chronic nasopharyngeal inflammatory tissue (20%), 10%(P0.05).HIF-2a protein expression and T stage. The expression of P0.05, GLUT-1 protein associated with cervical lymph node metastasis and clinical stage was significantly correlated with the clinical stage, and whether there was a significant correlation between the expression of P0.05 protein and the patient's sex, but the expression of both proteins was not related to the gender of the patient, nor was the expression of P0.05. OPN protein correlated with the sex of the patient. There was no significant correlation between age T stage, cervical lymph node metastasis and clinical stage (P 0.05). There was no positive correlation between the expression of HIF-2 伪 and GLUT-1 protein and the expression of HIF-2a and GLUT-1 in nasopharyngeal carcinoma. The expression intensity of HIF-2 伪 GLUT-1 and OPN during radiotherapy was significantly lower than that before radiotherapy. The serum OPN level in nasopharyngeal carcinoma group (161.02 卤44.80ng/ml) was significantly higher than that in normal control group (58.89 卤24.65 ng / ml), and the difference was statistically significant (P 0.05). Serum OPN level was correlated with clinical stage, cervical lymph node metastasis or not, but not with gender and age T stage. There was no correlation between the serum OPN level and the expression of HIF-2 伪 -GLUT-1 and OPN protein in nasopharyngeal carcinoma. Conclusion the overexpression of HIF-2 伪 -GLUT-1 and OPN protein plays an important role in the pathogenesis and development of nasopharyngeal carcinoma. The expression of HIF-2a and GLUT-1 is of great significance in clinical staging, evaluation of curative effect and prognosis of nasopharyngeal carcinoma. Serum OPN level can be used as an auxiliary index for diagnosis, staging and prognosis of nasopharyngeal carcinoma.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.63

【参考文献】