KAI1和COX-2在喉鳞癌组织中的表达及相关性研究

发布时间：2018-05-03 03:36

本文选题：喉鳞癌 + KAI1　；参考：《内蒙古医科大学》2011年硕士论文

【摘要】：目的了解KAI1和COX-2在喉鳞癌组织中的表达的情况,探讨二者在喉鳞癌中表达的意义及相关性，同时探讨二者在喉鳞状细胞癌发生发展及浸润转移过程中的作用。方法应用免疫组织化学SP法分别检测45例经病理证实喉鳞状细胞癌患者的咽喉黏膜组织、10例正常体检者咽喉黏膜组织中KAI1和COX-2的表达水平，分析二者表达水平及强度与病例的相关临床指标之间的关系，并分析这两个基因之间在喉鳞状细胞癌中表达的相关性。结果KAI1基因在喉鳞癌细胞中的表达低于在正常咽喉黏膜组织中的表达(χ~2=4.11, P 0.05)；在喉癌组织中表达的阳性率与年龄、性别、分化程度、临床分期及是否有淋巴结转移均无关(P0.05),但其阳性表达强度与临床分期及是否有淋巴结转移有显著相关性(P 0.05)。COX-2基因在喉鳞癌组织中高表达，在正常咽喉黏膜组织中低表达，差异有显著性(P 0.01),在喉鳞癌组织中表达的阳性率与性别、年龄及分化程度无关，而与临床分期及是否有淋巴结转移有显著相关性(P 0.05)。KAI1和COX-2在喉鳞癌组织中的表达呈负相关（关联系数r=-0.30,P 0.05)。结论KAI1的表达在喉鳞癌的发生及浸润转移过程中减弱或丢失，说明其在喉鳞癌的发生、发展过程中具有一定抑制作用，可作为今后预测肿瘤细胞转移潜能的指标；COX-2在喉鳞癌的发生、发展过程中表达增强，，同样说明其在喉鳞癌细胞的发生发展及浸润转移过程中起一定的促进作用，也可作为今后检测肿瘤细胞发生和转移潜能的指标之一。
[Abstract]:Objective to investigate the expression of KAI1 and COX-2 in laryngeal squamous cell carcinoma (LSCC) and to explore their significance and correlation in laryngeal squamous cell carcinoma (LSCC). Methods Immunohistochemical SP method was used to detect the expression of KAI1 and COX-2 in laryngeal mucosa of 45 patients with pathologically proved laryngeal squamous cell carcinoma (LSCC). To analyze the relationship between the expression level and intensity of the two genes and the clinical parameters of the patients, and to analyze the correlation between the expression of these two genes in laryngeal squamous cell carcinoma (LSCC). Results the expression of KAI1 gene in laryngeal squamous cell carcinoma cells was lower than that in normal laryngeal mucosa (蠂 ~ (2 +) 24.11, P 0.05), and the positive rate and age, sex, differentiation degree of KAI1 gene in laryngeal carcinoma tissue were significantly lower than those in normal laryngeal mucosa (蠂 ~ (2 +) 24.11, P 0.05). There was no correlation between clinical stage and lymph node metastasis, but there was a significant correlation between the positive expression of P 0.05).COX-2 gene and clinical stage and lymph node metastasis, and the low expression of P 0.05).COX-2 gene in normal pharynx and laryngeal mucosa. The positive rate of expression in laryngeal squamous cell carcinoma was not related to sex, age and degree of differentiation. The expression of P 0.05).KAI1 and COX-2 in laryngeal squamous cell carcinoma was negatively correlated with clinical stage and lymph node metastasis. Conclusion the expression of KAI1 is decreased or lost during the occurrence and invasion and metastasis of laryngeal squamous cell carcinoma, which indicates that it has a certain inhibitory effect on the occurrence and development of laryngeal squamous cell carcinoma. The expression of COX-2 in laryngeal squamous cell carcinoma (LSCC) may be used as a predictor of metastatic potential of tumor cells in the future, which also indicates that COX-2 plays an important role in the development, invasion and metastasis of laryngeal squamous cell carcinoma (LSCC). It can also be used as one of the indicators of tumor cell development and metastasis potential in the future.
【学位授予单位】：内蒙古医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65

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