新霉素致损小鼠听觉上皮后线粒体转录因子B1与毛细胞凋亡的关系

发布时间：2018-05-03 09:25

本文选题：TFBM + 线粒体　；参考：《第三军医大学学报》2015年02期

【摘要】：目的研究新霉素致损小鼠听觉上皮后线粒体转录因子B1(mitochodnrial transcription factor B1,TFB1M)与听觉上皮毛细胞凋亡的关系。方法每次解剖出20只出生1~2 d的新生ICR小鼠40个耳蜗,使用体外无血清培养法培养听觉上皮,培养后分为正常对照组和新霉素致损组(n=20),然后在新霉素致损4、12、24 h及24 h洗脱后2 d检测TFB1M在听觉上皮细胞核和线粒体中的表达,以及听觉上皮线粒体膜电位的变化和毛细胞的凋亡。结果新霉素致损4 h后,听觉上皮中圈毛细胞形态完整,其线粒体膜电位开始降低;致损12 h,耳蜗听觉上皮线粒体以及细胞核内的TFB1M表达上调,听觉上皮出现毛细胞底圈、中圈大量毛细胞缺失,未缺失处毛细胞出现线粒体膜电位明显降低;致损24 h中圈、底圈毛细胞基本消失,未检测到线粒体膜电位。洗脱后2 d,TFB1M表达较正常对照组降低,基底膜毛细胞未见进一步缺失。而正常对照组在新霉素致损组相对应的时间点上,离体培养的听觉上皮的毛细胞形态完整,排列整齐,线粒体膜电位无改变。新霉素致损12 h,TFB1M在线粒体和细胞核内的表达较4、24 h,洗脱后2 d都明显(P0.01)。结论 TFB1M蛋白质水平的表达上调可能导致线粒体功能异常和毛细胞凋亡。
[Abstract]:Objective to study the relationship between the mitochondrial transcription factor B1(mitochodnrial transcription factor B1T TFB1M and the apoptosis of epithelial cells in auditory epithelium of mice induced by neomycin. Methods 40 cochleas of 20 newborn ICR mice were dissected at 1d and 2d each time, and the auditory epithelium was cultured by serum-free culture in vitro. After culture, they were divided into normal control group and neomycin damaged group. The expression of TFB1M in the nucleus and mitochondria of auditory epithelium was detected at 24 h and 24 h after neomycin damage. The changes of mitochondrial membrane potential and the apoptosis of hair cells in auditory epithelium. Results after 4 h of neomycin damage, the morphology of hair cells in the middle circle of auditory epithelium was intact, the mitochondrial membrane potential began to decrease, and the expression of TFB1M in mitochondria and nucleus of cochlea auditory epithelium was up-regulated at 12 h, and the basal loop of hair cell appeared in auditory epithelium. In the middle circle, a large number of hair cells were absent, and the mitochondrial membrane potential was decreased in the hair cells without loss, while in the middle circle after 24 h damage, the basal hair cells disappeared, and no mitochondrial membrane potential was detected. 2 d after elution, the expression of TFB1M was lower than that of normal control group, and no further loss of basal membrane hair cells was found. In the normal control group, in the corresponding time point of neomycin induced damage group, the hair cells of the cultured auditory epithelium in vitro were intact, arranged neomycin damage group, and the mitochondrial membrane potential was not changed. The expression of TFB1M in mitochondria and nucleus was significantly higher than that in mitochondria and nucleus at 12 h after neomycin was damaged, and the expression of TFB1M in mitochondria and nucleus was significantly increased 2 days after elution. Conclusion the up-regulation of TFB1M protein may lead to mitochondrial dysfunction and hair cell apoptosis.
【作者单位】：重庆医科大学附属儿童医院耳鼻喉科;复旦大学附属眼耳鼻喉科医院耳鼻喉科;
【基金】：重庆市科委自然科学基金(CSTC2010BB537)~~
【分类号】：R764.43

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