损毁豚鼠一侧耳蜗后水杨酸钠给药对对侧耳螺旋神节GLU、GABA表达的影响

发布时间：2018-05-04 04:44

本文选题：水杨酸钠 + 豚鼠　；参考：《广西医科大学》2011年硕士论文

【摘要】：目的:观察损毁豚鼠一侧耳蜗后水杨酸钠给药对对侧耳螺旋神经节GLU、GABA表达的影响方法:出生后体重在250～300g的成年豚鼠64只随机分成四组,每组16只。A组:正常对照组;B组:单纯手术组;C组:单纯水杨酸钠给药组300mg/(kg.d) D组:一侧耳蜗捣毁+水杨酸钠给药[300mg/(kg.d)]组A组和B组经腹腔给与同等计量的生理盐水。分别在给药15天及停药15天后处死动物,制备耳蜗标本,通过免疫组织化学染色检测对侧耳蜗GABA、GLu在螺旋神经节的表达。所有动物术后均经后腿肌肉注射青霉素钠预防感染(注射剂量为:160万U/kg/d,1次/天,共给药1周)。结果:(1):给药15d后,C组(水杨酸钠给药组)右侧耳蜗螺旋神经节GLU阳性细胞明显高于A组(正常对照组)和B组(P㩳0.001,P㩳0.001);A组和B组比较无统计学意义。 D组(耳蜗捣毁+水杨酸钠给给药)右侧耳蜗螺旋神经节GLU阳性细胞表达与A组和B组比较(P㩳0.001,P㩳0.001)有显著差异;D组与C组比较P㧐0.05,无统计学意义。 (2):停药15天后, C组(水杨酸钠给药组)右侧耳蜗螺旋神经节GLU阳性细胞亦明显高于A组(正常对照组)和B组(P㩳0.05,P㩳0.01);A组和B组比较无统计学意义。 D组(耳蜗捣毁+水杨酸钠给给药)右侧耳蜗螺旋神经节GLU阳性细胞表达与A组和B组比较(P㩳0.001,P㩳0.001)有显著差异;D组与C组比较P㩳0.05,有统计学意义. (3):给药15d后,C组(水杨酸钠给药组)右侧耳蜗螺旋神经节GABA阳性细胞明显低于A组(正常对照组)和B组(P㩳0.05,P㩳0.05);A组和B组比较无统计学意义。 D组(耳蜗捣毁+水杨酸钠给给药)右侧耳蜗螺旋神经节GABA阳性细胞表达与A组和B组比较(P㩳0.01,P㩳0.01)有显著差异;D组与C组比较P㧐0.05,无统计学意义。 (4):停药15天后, C组(水杨酸钠给药组)右侧耳蜗螺旋神经节GABA阳性细胞与A组(正常对照组)和B组比较无统计学意义;A组和B组比较亦无统计学意义。 D组(耳蜗捣毁+水杨酸钠给给药)右侧耳蜗螺旋神经节GABA阳性细胞表达与A组和B组比较无显著差异;D组与C组比较亦无统计学意义。结论:1.一侧耳蜗捣毁+水杨酸钠给给药能使对侧耳GLU阳性细胞表达明显上调,停药15天后这种改变仍然存在。 2.一侧耳蜗捣毁+水杨酸钠给给药能明显下调对侧耳GABA阳性细胞表达,停药15天后这种改变消失。
[Abstract]:Objective: to observe the effect of sodium salicylate on the expression of GLU in spiral ganglion of lateral ear after unilateral cochlear lesion in guinea pigs. Methods: Sixty-four adult guinea pigs weighing 250 ~ 300g after birth were randomly divided into four groups. 16 rats in each group: normal control group, group B: simple operation group, group C: sodium salicylate group, 300mg / kg 路d) D group: unilateral cochlea destroyed salicylate [300mg / kg 路d] group A and B were given intraperitoneally the same amount of normal saline. Cochlear specimens were prepared after 15 days of administration and 15 days after withdrawal. The expression of GABAA-GLu in the spiral ganglion of the contralateral cochlea was detected by immunohistochemical staining. All animals were injected penicillin sodium intramuscularly after operation to prevent infection (1.6 million U / kg 路d ~ (-1) 路d ~ (-1) 路d ~ (-1) 路d ~ (-1)) for one week. Results GLU positive cells in right cochlear spiral ganglion in group C (sodium salicylate group) were significantly higher than those in group A (normal control group) and group B (group B). There was no significant difference between group A and group B. The expression of GLU positive cells in the right cochlear spiral ganglion in group D (treated with sodium salicylate) was significantly different from that in group A and group B. There was no significant difference between group D and group C (P < 0.05). GLU positive cells in right cochlear spiral ganglion in group C (sodium salicylate administration group) were significantly higher than those in group A (normal control group) and group B (group B). There was no significant difference between group A and group B. The expression of GLU positive cells in the right cochlear spiral ganglion in group D (treated with sodium salicylate) was significantly different from that in group A and group B. there was a significant difference between group D and group C (P < 0.05). GABA positive cells in right cochlear spiral ganglion in group C (sodium salicylate group) were significantly lower than those in group A (normal control group) and group B (group B). There was no significant difference between group A and group B. The expression of GABA positive cells in the right cochlear spiral ganglion in group D (treated with sodium salicylate) was significantly different from that in group A and group B. There was no significant difference between group D and group C (P < 0.05). GABA positive cells in right cochlear spiral ganglion in group C (sodium salicylate) were not significantly different from those in group A (normal control group) and group B (control group). There was no significant difference in the expression of GABA positive cells in right cochlear spiral ganglion between group D and group A and group B. there was no significant difference between group D and group C. Conclusion 1. Sodium salicylate on one side of the cochlea could significantly up-regulate the expression of GLU positive cells in the contralateral ear, and the change still existed 15 days after withdrawal. 2. Sodium salicylate on one side of the cochlea could significantly down-regulate the expression of GABA positive cells in the contralateral ear, and the change disappeared 15 days after withdrawal.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R764

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