姜黄素眼用脂质体的制备及晶状体抗氧化作用考察

发布时间：2018-05-05 00:24

本文选题：姜黄素 + N-三甲基壳聚糖　；参考：《中国实验方剂学杂志》2014年06期

【摘要】：目的:制备N-三甲基壳聚糖(TMC)包覆姜黄素眼用脂质体并考察其抑制大鼠硒性白内障晶状体氧化作用。方法:采用乙醇注入法制备姜黄素脂质体(Cur-TCL),通过星点设计-效应面法优化脂质体处方工艺;采用过膜法、透射电镜、粒径电位测定仪测定Cur-TCL的理化性质;建立大鼠硒性白内障模型,考察Cur-TCL对白内障形成过程中晶状体氧化的抑制作用。结果:最佳处方为姜黄素1.41 mg,磷脂质量分数4%,胆固醇-磷脂(1∶5.44)。经0.5%TMC溶液包覆后脂质体呈球形,粒径增大,电位显著提高,药物包封率基本不变。与未包覆脂质体相比,Cur-TCL组大鼠晶状体中SOD活力提高30.3%,MDA摩尔浓度减少52.4%,GSH含量增加1.29倍。结论:Cur-TCL可显著抑制硒性白内障导致的晶状体氧化过程,为该制剂治疗白内障提供参考。
[Abstract]:Aim: to prepare N-trimethyl chitosan (TMC) coated curcumin liposome and investigate its effect on inhibiting the oxidation of rat selenium-induced cataract lens. Methods: curcumin liposome Cur-TCLL was prepared by ethanol injection. The prescription of curcumin liposome was optimized by star design-effect surface method, and the physicochemical properties of Cur-TCL were determined by overmembrane method, transmission electron microscope and particle size potentiometer. To investigate the inhibitory effect of Cur-TCL on lens oxidation during cataract formation in rats. Results: the best prescription was curcumin 1.41 mg, mass fraction of phospholipid 4 and cholesterol-phospholipid 1: 5.44%. After coated with 0.5%TMC solution, the liposome was spherical, the particle size was increased, the potential was significantly increased, and the encapsulation efficiency of the drug was almost unchanged. Compared with uncoated liposome, the activity of SOD in the lens of rats in Cur-TCL group was increased by 30.3%. The content of SOD in rat lens was decreased by 52.4 times than that in uncoated liposome group, and the content of SOD was increased by 1.29 times. Conclusion 1: Cur-TCL can significantly inhibit the process of lens oxidation induced by selenium cataract and provide reference for the treatment of cataract.
【作者单位】：江西中医药大学现代中药制剂教育部重点实验室;江西中医药大学中药固体制剂制造技术国家工程研究中心;江西中医药大学药学院;
【基金】：国家自然科学基金项目(81202927) 江西省研究生创新专项基金项目(YC2012-S106) 江西省教育厅青年基金项目(GJJ12535,GJJ12536) 江西省卫生厅中医药科研计划(普通)课题(2012A160,2012A157) 江西省青年科学基金项目(20132BAB215022)
【分类号】：R776.1

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