Neuropilin-2在喉癌组织中的表达及其临床意义

发布时间：2018-05-06 02:40

本文选题：NRP-2 + VEGF　；参考：《中南大学》2014年硕士论文

【摘要】：目的：检测Neuropilin-2(神经菌毛蛋白2,简称NRP-2)在喉癌组织中的表达,并初步研究其表达水平的高低与喉癌各不同临床病理特征及预后之间的相互关系。方法：采用免疫组织化学S-P法检测NRP-2蛋白在70例喉癌石蜡组织标本中的表达,并运用统计学方法分析NRP-2蛋白表达水平与喉癌各不同临床病理特征及预后之间的相互关系;同时,通过Western blot检测NRP-2蛋白在15例喉癌及相应癌旁黏膜组织中的表达情况。结果：NRP-2蛋白的表达高低与患者的年龄、性别、病变部位、TNM分期、临床分期以及淋巴结转移等无显著的相关性,与病变组织的病理学分级呈显著的相关性,P0.05。蛋白质印迹实验显示NRP-2在癌旁组织的表达,明显低于相配对的癌组织,差别具有统计学意义,P0.05。通过Kaplan-Meier分析,NRP-2高表达组患者的五年生存率及无复发生存率与NRP-2低表达组患者之间的差别不具有统计学意义,P0.05,但NRP-2的表达水平的高低与患者喉癌预后的差异具有显著性差异,P0.05。结论：NRP-2蛋白在喉癌组织中表达显著升高,且其高表达与喉癌患者病变组织的病理学分级呈显著的相关性。NRP-2的表达可能在喉癌的发生、生长及转移中起重要作用,在一定程度上,可作为检测喉癌的复发,判断预后的指标。
[Abstract]:Aim: to detect the expression of Neuropilin-2 (NRP-2) in laryngeal carcinoma and to study the relationship between the expression level and the clinicopathological features and prognosis of laryngeal carcinoma. Methods: immunohistochemical S-P method was used to detect the expression of NRP-2 protein in 70 paraffin tissues of laryngeal carcinoma. The correlation between the expression of NRP-2 protein and the clinicopathological features and prognosis of laryngeal carcinoma was analyzed by statistical method. Western blot was used to detect the expression of NRP-2 protein in 15 cases of laryngeal carcinoma and its adjacent mucosa. Results there was no significant correlation between the expression of WNRP-2 protein and age, sex, TNM stage, clinical stage and lymph node metastasis, but there was a significant correlation between the expression of the protein and pathological grade of the lesion (P 0.05). Western blot analysis showed that the expression of NRP-2 in paracancerous tissues was significantly lower than that in matched tissues (P 0.05). The 5-year survival rate and recurrence free survival rate of patients with high expression of NRP-2 were not significantly different from those of patients with low expression of NRP-2 by Kaplan-Meier analysis (P 0.05), but the level of NRP-2 expression was significantly different from the prognosis of laryngeal carcinoma (P 0.05). Conclusion the high expression of the protein is significantly correlated with the pathological grade of laryngeal carcinoma. The expression of NRP-2 may play an important role in the occurrence, growth and metastasis of laryngeal carcinoma, and may play an important role in the development, growth and metastasis of laryngeal carcinoma to a certain extent, and the expression of NRP-2 may play an important role in the development, growth and metastasis of laryngeal carcinoma. It can be used as an index to detect the recurrence of laryngeal carcinoma and to judge the prognosis.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.65

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