2,3,7,8-四氯二苯-对-二恶英对小鼠实验性过敏性结膜炎抑制作用的实验研究

发布时间：2018-05-06 06:04

本文选题：过敏性结膜炎 + -四氯二苯-对-二恶英　；参考：《眼科》2015年02期

【摘要】：目的研究2,3,7,8,-四氯二苯-对-二恶英(2,3,7,8-Tetrachlor odibenzo-p-dioxin,TCDD)对实验性过敏性结膜炎的抑制作用。设计实验研究。研究对象24只雌性BALB/c小鼠随机分成2组,TCDD组和对照组各12只。方法用豚草花粉(Ragweed,RW)对BAL B/c小鼠免疫的前1天,小鼠腹腔内注射1μg TCDD(TCDD组),对照组注射等量的橄榄油,免疫后的第10天用含有RW的溶液点眼攻击,观察眼部临床表现(眼睑充血水肿、流泪),攻击后24小时将小鼠处死,取出眼球及上下眼睑,进行病理分析,计数嗜酸性粒细胞浸润的个数;取出脾脏分离淋巴细胞,分别用特异性抗原Ragweed及Anti-CD3m Ab刺激,通过酶联免疫吸附实验检测细胞因子(IL-4、IL-5、IFN-γ、IL-10)的生成,以3H-Td R掺入法通过液体闪烁计数仪测定每分钟脉冲数值检测细胞增生反应。主要指标结膜炎临床与病理表现(眼睑充血水肿、流泪,嗜酸性粒细胞浸润的个数),IL-4、IL-5、IFN-γ、IL-10细胞因子数,T淋巴细胞增生反应数。结果 TCDD组未发现明显的临床症状,而对照组小鼠出现不同程度流泪、结膜充血等症状。TCDD组脾细胞中未测到IL-4及IL-5生成,对照组IL-4及IL-5分别为(5.34±0.82)pg/ml和(3.56±0.11)pg/ml(t=2.3,P=0.025);但经过Anti-CD3m Ab刺激后TCDD组细胞因子IL-4及IL-5分别为(2.31±0.49)pg/ml和(3.16±0.66)pg/ml,对照组为(2.82±1.12)pg/ml和(4.31±0.89)pg/m l(t=0.15,P=0.078);TCDD组及对照组小鼠脾细胞在经过RW抗原刺激后细胞增生反应分别为(300±13)cpm和(1500±15)cpm(P=0.01),而在抗Anti-CD3m Ab刺激后细胞增生为(15 500±550)cpm和(16 700±670)cpm,差异无统计学意义(P=0.071)。结论 TCDD抑制了小鼠实验性过敏性结膜炎的发展。
[Abstract]:Objective to study the inhibitory effect of TCDDD on experimental allergic conjunctivitis. Design experimental research. Participants 24 female BALB/c mice were randomly divided into two groups: TCDD group (n = 12) and control group (n = 12). Methods the mice were injected intraperitoneally with 1 渭 g TCDD(TCDD on the first day before immunization with Ragweeden (RW), and the control group was injected with the same amount of olive oil. On the 10th day after immunization, the mice were attacked with RW solution. Observe the clinical manifestations of the eyes (hyperemia and edema of eyelid, tears, 24 hours after attack, kill the mice, take out the eyeballs and upper and lower eyelids, carry out pathological analysis, count the number of eosinophils infiltrating, remove the lymphocytes isolated from the spleen, Specific antigen Ragweed and Anti-CD3m Ab were used to detect the production of IL-4 and IL-5IFN- 纬 IL-10 by enzyme-linked immunosorbent assay (Elisa). The proliferation of cells was measured by 3H-Td R incorporation and pulse per minute by liquid scintillation counter. Main outcome measures: clinical and pathological manifestations of conjunctivitis (blepharohyperemia and edema, tears, eosinophilic granulocyte infiltration) and the number of IL-4, IL-5, IFN- 纬 -IL-10 cytokines and the number of T lymphocyte proliferative responses. Results No obvious clinical symptoms were found in the TCDD group, while in the control group, tears occurred in different degrees, and no IL-4 and IL-5 production was detected in the spleen cells of the TCDD group, such as conjunctival hyperemia. The IL-4 and IL-5 of the control group were 5.34 卤0.82)pg/ml and 3.56 卤0.11pgml / ml respectively, but the cytokines IL-4 and IL-5 of the TCDD group were 2.31 卤0.49)pg/ml and 3.16 卤0.66 PG / ml, respectively, while those of the control group and the control group were 2.82 卤1.12)pg/ml and 4.31 卤0.89)pg/m ~ 0.15 ~ 0.78% respectively, and that of the control group and the control group were 2.31 卤0.49)pg/ml and 3.16 卤0.66 渭 g / ml, respectively, and those of the control group and the control group were respectively stimulated by RW antigen. There was no significant difference in the proliferation of cells induced by Anti-CD3m Ab (15 500 卤550)cpm) and 16 700 卤6 70 cpm (P < 0. 071). Conclusion TCDD inhibits the development of experimental allergic conjunctivitis in mice.
【作者单位】：内蒙古自治区人民医院眼科;
【分类号】：R777.31

【共引文献】