Serglycin稳定干扰增加鼻咽癌高转移细胞对顺铂的敏感性

发布时间：2018-05-06 07:06

  本文选题：serglycin + 鼻咽癌　； 参考：《中国药学杂志》2015年08期

【摘要】：目的观察serglycin稳定干扰后对鼻咽癌高转移细胞顺铂敏感性的影响,并初步探究其机制。方法建立serglycin稳定干扰的鼻咽癌高转移细胞株,qRT-PCR和Western blot检测serglycin的表达情况。四甲基偶氮唑蓝法测定顺铂对稳定株细胞的IC50;增殖曲线实验观察在顺铂压力下细胞增殖的差异;克隆形成实验观察细胞克隆形成能力的差异;Annexin V/PI双染检测细胞凋亡。qRT-PCR检测稳定株细胞中干性基因的表达情况。结果 Serglycin稳定干扰的鼻咽癌高转移细胞株成功建立,细胞形态发生改变。四甲基偶氮唑蓝法测得serglycin稳定干扰细胞的顺铂IC50显著低于对照组细胞,增殖抑制率高于对照组,药物作用下克隆形成能力减弱,流式凋亡比例增高。Serglycin稳定干扰后干性基因表达下调。结论 Serglycin稳定干扰可以增加鼻咽癌高转移细胞对顺铂的敏感性。
[Abstract]:Objective to investigate the effect of serglycin stable interference on cisplatin sensitivity of nasopharyngeal carcinoma (NPC) cells with high metastasis. Methods the expression of serglycin was detected by qRT-PCR and Western blot in nasopharyngeal carcinoma (NPC) cell lines with stable interference of serglycin. The IC50 of cisplatin on stable cell line was determined by tetramethyl azolium blue method, and the difference of cell proliferation under the pressure of cisplatin was observed by the proliferation curve experiment. Clone formation assay was used to observe the difference of cell clone forming ability. Annexin V/PI double staining was used to detect apoptosis. QRT-PCR was used to detect the expression of dry genes in stable cell lines. Results the high metastatic nasopharyngeal carcinoma cell line with stable interference of Serglycin was successfully established and the morphology of the cell was changed. The cisplatin IC50 of serglycin stable interfering cells was significantly lower than that of control cells, and the inhibition rate of proliferation was higher than that of control group. The expression of dry genes was down-regulated after steady interference of apoptosis with increased flow rate. Conclusion stable interference of Serglycin can increase the sensitivity of high metastatic nasopharyngeal carcinoma cells to cisplatin.
【作者单位】： 中山大学附属肿瘤医院 华南肿瘤学国家重点实验室;
【基金】：广东省科技计划项目(2012B020306001)
【分类号】：R739.63
，

本文编号：1851296