FLNA在鼻咽癌发生中的作用及其可能的分子机制

发布时间：2018-05-06 20:44

本文选题：细丝蛋白A + 鼻咽肿瘤　；参考：《中国肿瘤生物治疗杂志》2014年05期

【摘要】：目的:探讨细丝蛋白A(filamin A,FLNA)在鼻咽癌中的表达水平及其过表达对鼻咽癌(nasopharyngeal carcinoma,NPC)细胞生物表型的影响。方法:收集2008年1月至2008年12月唐山市人民医院病理科活检取得并经病理证实的新鲜NPC组织标本63例及21例距其癌组织边缘2 cm以上且镜下未见癌浸润的正常鼻咽组织(对照组),采用免疫组织化学及Western blotting方法检测NPC组织及正常鼻咽组织中FLNA及MMP-9蛋白的表达。以慢病毒转染建立FLNA过量表达的NPC CNE2细胞株,采用RT-PCR及Western blotting检测转染后NPC CNE2细胞株中FLNA的表达变化;MTT法检测FLNA蛋白过量表达对NPC细胞增殖的影响,Transwell实验检测CNE2细胞的侵袭能力。结果:FLNA蛋白在NPC组织中的阳性表达率明显低于正常鼻咽组织(36.5%vs 66.7%,P0.05),其在NPC组织的相对表达量较正常鼻咽组织的相对表达量明显降低[(0.378±0.031)vs(0.835±0.078),P0.05],FLNA蛋白的表达水平与NPC T分期、有无淋巴结转移、临床分期以及组织分级有关(P0.05)。FLNA蛋白高表达的CNE2细胞其增殖能力明显减弱、侵袭转移能力明显降低、MMP-9蛋白表达量明显下调。结论:NPC组织中FLNA蛋白表达明显降低可能是鼻咽黏膜恶性转变的重要生物学标志,对预测NPC发生、浸润、转移有重要意义。
[Abstract]:Aim: to investigate the expression level of A(filamin Arona in nasopharyngeal carcinoma (NPC) and the effect of overexpression on the biological phenotype of nasopharyngeal carcinoma (NPC) cells. Methods: from January 2008 to December 2008, 63 specimens of fresh NPC tissues obtained from pathology department of people's Hospital of Tangshan City and 21 cases of normal nose which were more than 2 cm from the margin of cancer tissue and were not infiltrated under microscope were collected. The expression of FLNA and MMP-9 in NPC and normal nasopharyngeal tissues were detected by immunohistochemistry and Western blotting. The FLNA overexpressed NPC CNE2 cell lines were established by lentivirus transfection. The changes of FLNA expression in NPC CNE2 cell lines after transfection were detected by RT-PCR and Western blotting. The effect of FLNA overexpression on the proliferation of NPC cells was detected by MTT assay. The invasion ability of CNE2 cells was detected by transwell assay. Results the positive expression rate of NPC was significantly lower than that of normal nasopharyngeal tissue (36.5 vs 66.7), and the relative expression level in NPC was significantly lower than that in normal nasopharyngeal tissue [0.378 卤0.031)vs(0.835 卤0.078] and the NPC T stage was significantly lower than that in normal nasopharyngeal tissue. With or without lymph node metastasis, the proliferative ability of CNE2 cells with high expression of P0.05, FLNA protein and invasion and metastasis were significantly decreased, and the expression of MMP-9 protein was significantly down-regulated. Conclusion the decreased expression of FLNA protein may be an important biological marker for malignant transformation of nasopharyngeal mucosa, and may be of great significance in predicting the occurrence, invasion and metastasis of NPC.
【作者单位】：唐山市人民医院放化疗科;唐山市人民医院病理科;河北医科大学第四医院放疗科;
【分类号】：R739.63

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