噪声性听力损伤对豚鼠耳蜗Prestin蛋白及mRNA表达水平影响

发布时间：2018-05-07 05:54

本文选题：Prestin蛋白 + Prestin　；参考：《中国职业医学》2014年04期

【摘要】：目的探讨噪声性听力损伤(NIHL)对耳蜗Prestin蛋白表达的影响。方法无特定病原体级健康雄性豚鼠60只随机分为对照组和85、95、105、115 dB声压级(SPL)暴露组5组,除对照组外,其余4组分别予以85、95、105、115 dB SPL的高斯白噪声暴露,每天6 h,连续28 d,暴露前1 d和暴露结束后第7天分别进行听性脑干反应测量,噪声暴露结束后第7天,分别进行耳蜗病理学检查、逆转录-聚合酶链反应分析Prestin mRNA表达水平、蛋白免疫印迹法分析Prestin蛋白表达水平。结果对照组和85、95、105、115 dB SPL暴露组的永久性听阈位移(PTS)水平依次为(2.08±0.97)、(7.08±2.79)、(13.33±1.63)、(21.88±1.88)和(27.92±2.34)dB nHL,上述5组PTS水平两两比较,差异均有统计学意义(P0.05)。病理学检查结果显示115 dB SPL暴露组豚鼠耳蜗出现明显毛细胞损伤。上述5组Prestin mRNA的2-ΔΔCt值依次为(1.12±0.39)、(0.98±0.24)、(1.53±0.55)、(2.84±0.51)和(7.65±0.63);105 dB SPL暴露组Prestin mRNA的2-ΔΔCt值分别高于对照组及85、95 dB SPL暴露组(P0.05),115 dB SPL暴露组Prestin mRNA的2-ΔΔCt值分别高于其余4组(P0.05)。上述5组Prestin蛋白相对灰度值依次为(0.18±0.07)、(0.20±0.09)、(0.29±0.10)、(0.45±0.19)和(1.19±0.16);105 dB SPL暴露组Prestin蛋白相对灰度值分别高于对照组及85、95 dB SPL暴露组(P0.05),115 dB SPL暴露组Prestin蛋白相对灰度值分别高于其余4组(P0.05)。结论严重的NIHL会导致耳蜗外毛细胞Prestin的表达代偿性增高。
[Abstract]:Objective to investigate the effect of noise-induced hearing impairment (NIHLL) on the expression of Prestin protein in cochlea. Methods 60 healthy male guinea pigs without specific pathogens were randomly divided into five groups: control group (n = 5) and control group (n = 5). Except for the control group, the other four groups were exposed to Gao Si white noise (Gao Si) of 95105115 dB SPL. Auditory brainstem response was measured 1 day before exposure and 7 days after exposure. Cochlea pathological examination and reverse transcription-polymerase chain reaction (RT-PCR) were performed on the 7th day after noise exposure. The expression of Prestin protein was analyzed by Western blot. Results the levels of permanent auditory threshold shift (PTSs) in the control group and the 85 + 95 105115 dB SPL exposure group were 2.08 卤0.97, 7.08 卤2.79, 13.33 卤1.63, 21.88 卤1.88) and 27.92 卤1.88, respectively. There were significant differences in PTS levels between the five groups (P 0.05). Histopathological examination showed that the cochlea of guinea pigs exposed to 115dB SPL showed obvious hair cell injury. The 2- 螖 Ct values of Prestin mRNA in the above five groups were 1.12 卤0.39, 0.98 卤0.24, 1.53 卤0.55, 2.84 卤0.51) and 7.65 卤0.63 ~ 105dB SPL, respectively, which were higher than those in the control group and the 8595 dB SPL group, respectively. The values of Prestin mRNA in the SPL group were higher than those in the other four groups (P 0.05). The relative gray values of Prestin protein in the above five groups were 0.18 卤0.07dB SPL 0.20 卤0.09 SPL 0.29 卤0.10fen 0.45 卤0.19) and 1.19 卤0.16105dB SPL, respectively, which were higher than those in the control group and 8550dB SPL group, respectively. The relative gray value of Prestin protein in the group exposed to SPL was higher than that in the other four groups. Conclusion severe NIHL may lead to compensatory increase of Prestin expression in cochlear outer hair cells.
【作者单位】：广东药学院公共卫生学院;广东省分子流行病学重点实验室;
【基金】：广东省职业病防治重点实验室开放课题基金(2012A061400007-01)
【分类号】：R764

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