原发性开角型青光眼小梁网组织早衰的初步研究

发布时间：2018-05-07 12:56

本文选题：原发性开角型青光眼 + 小梁网组织　；参考：《中山大学学报(医学科学版)》2014年05期

【摘要】：【目的】观察正常小梁网组织和POAG小梁网组织在超微结构和衰老相关β-半乳糖苷酶(SA-β-Gal)表达方面是否存在差异。【方法】正常人小梁网组织取自广东省眼库的正常人眼球(10例),平均年龄(36.90±10.10)岁;POAG小梁网组织来自中山眼科中心青光眼科行小梁切除术者(10例),平均年龄(39.80±11.64)岁。用透射电镜观察两组小梁网组织超微结构;用衰老相关β-半乳糖苷酶(SA-β-Gal)染色法对两组小梁网组织进行染色,并分析两者之间衰老染色的差异。【结果】透射电镜发现正常人小梁网组织细胞膜完整,核染色均匀居中,胞浆中可见形态正常的中心体,周围散落分布着的线粒体、溶酶体等细胞器;基底膜完整连续;弹性纤维周围绕有薄鞘,胶原纤维呈束状排列、整齐;Schlemm管管腔通畅。而POAG患者的小梁网组织中可见细胞脱落溶解,细胞膜不完整,细胞核固缩或肿胀,线粒体水肿,线粒体嵴扩张;Schlemm管管腔变狭窄;弹性纤维增多且排列紊乱;基底膜增厚。SA-β-Gal染色发现POAG小梁网中的衰老细胞阳性率较正常人小梁网组织高(P0.001)。【结论】POAG小梁网超微结构破坏明显,SA-β-Gal染色增强,在POAG小梁网中可能存在早衰。
[Abstract]:[objective] to observe the difference between normal trabecular meshwork and POAG trabecular reticulum in ultrastructure and senescence related 尾 -galactosidase SA- 尾 -Gal.Methods the normal trabecular meshwork was taken from the eye bank of Guangdong Province. There were 10 cases (mean age: 36.90 卤10.10) years old (mean age: 36.90 卤10.10) of the trabeculectomy in 10 patients with Ophthalmology. The average age was 39.80 卤11.64 years old in 10 patients with trabeculectomy in Qingguang Eye Center of Zhongshan Ophthalmology Center. The ultrastructure of trabecular meshwork in two groups was observed by transmission electron microscope, and the tissue of trabecular meshwork was stained with senescence associated 尾 -galactosidase SA- 尾 -Gal) method. The difference of aging staining between them was analyzed. [results] Transmission electron microscope showed that the cell membrane of normal human trabecular meshwork was intact, the nucleus staining was uniform and the centrosome was seen in the cytoplasm, and the mitochondria were scattered around the cytoplasm. Lysosomes and other organelles; the basement membrane is complete and continuous; the elastic fibers are surrounded by thin sheath, the collagen fibers are arranged in bundles, and the Schlemm tube lumen is unobstructed. However, in the trabecular meshwork of POAG patients, the cells were exfoliated and dissolved, the cell membrane was incomplete, the nucleus was pyknosis or swelling, the mitochondria edema, the mitochondrial ridge dilated and the Schlemm tube lumen became narrow, the elastic fibers were increased and disordered. The positive rate of senescent cells in POAG trabecular meshwork was higher than that in normal human trabecular meshwork tissue by SA- 尾 -Gal staining. [conclusion] the ultrastructural damage of POAG trabecular meshwork was obviously enhanced by SA- 尾 -Gal staining, and there might be premature senescence in POAG trabecular meshwork.
【作者单位】：深圳市罗湖区人民医院;中山大学中山眼科中心//眼科学国家重点实验室;
【基金】：广东省医学科研基金(B2013139) 广州市科技计划(2013Z4500019) 佛山市医学类科技攻关项目(201308098)
【分类号】：R775

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