裸鼠喉癌细胞系Hep-2移植瘤中STK15基因的表达及意义

发布时间：2018-05-09 03:10

本文选题：裸鼠 + 喉癌细胞　；参考：《中国医科大学》2010年硕士论文

【摘要】： 实验目的观察裸鼠喉癌细胞系Hep-2移植瘤的成瘤率,绘制肿瘤生长曲线,用免疫组化方法观察移植瘤中STK15基因的表达情况。实验方法将符合条件的17只nu/nu纯系裸小鼠随机分成两组,实验组10只,对照组7只。实验组每只裸鼠右侧前肢腋下皮下注射0.2mlHep-2细胞悬液,直至皮下形成0.5cm大小皮丘。对照组每只裸鼠相同部位注射0.2ml生理盐水,直至皮下形成0.5cm大小皮丘,定期观察两组裸鼠接种部位液体的吸收情况,计算成瘤率,绘制成瘤曲线。5周后处死实验动物,剥离肿瘤,用免疫组化方法观察移植瘤中STK15基因的表达情况。实验结果实验组裸鼠成瘤率100%,免疫组化可证实裸鼠喉癌细胞系Hep-2移植瘤中STK15过表达。实验结论通过动物实验证实STK15基因是喉癌的发生发展的原因之一。
[Abstract]:experimental purpose

The tumor growth curve was observed and the expression of STK15 gene in transplanted tumor was observed by immunohistochemistry .

experimental method

Two groups of nude mice were randomly divided into two groups , the experimental group was 10 rats and the control group was 7 . The experimental group injected 0.2 ml of normal saline at the same location on the right anterior limb of the nude mice , and then injected 0.2ml normal saline at the same site of the nude mice . The tumor rate was calculated and the tumor rate was calculated . After 5 weeks , the experimental animals were sacrificed and the tumor was stripped . The expression of STK15 gene was observed by immunohistochemistry .

experimental results

The tumor rate of nude mice in experimental group was 100 % , and the expression of STK15 was confirmed by immunohistochemistry .

experimental conclusion

Animal experiments prove that the STK15 gene is one of the causes of the development of laryngeal cancer .

【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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