用聚合酶链反应（PCR）法病因诊断非典型临床表现的急性结膜炎研究

发布时间：2018-05-10 06:50

  本文选题：PCR法 + 结膜炎　； 参考：《延边大学》2011年硕士论文

【摘要】：目的：通过对非典型急性结膜炎患者结膜囊分泌物进行DNA提取以及PCR扩增,探讨聚合酶链反应法在非典型临床表现的急性结膜炎病因诊断中的临床意义。 方法：选择典型的急性细菌性结膜炎患者22人(22眼),典型急性病毒性结膜炎患者22人(22眼),典型过敏性结膜炎患者23人(23眼),非典型急性结膜炎患者44人(44眼),正常人22人(22眼),两眼共患结膜炎者取较重的一侧。所有受试者结膜囊分泌物提取的DNA分成两部分,分别加入细菌引物及腺病毒引物进行两次PCR扩增,凝胶电泳后在凝胶成像分析系统中观察结果。对于非典型急性结膜炎组患者同时进行细菌培养观察结果。 结果：1.典型急性细菌性结膜炎组(Ⅰ)有9眼扩增出520bp大小条带,阳性率40.9%;2眼扩增出1004bp条带,阳性率9.0%;520bp兼1004bp条带有1眼,阳性率4.54%。典型急性病毒性结膜炎组(Ⅱ)有2眼扩增出520bp大小条带,阳性率9.0%;8眼扩增出1004bp条带,阳性率36.3%;520bp兼1004bp条带有1眼,阳性率4.54%。非典型急性结膜炎组(Ⅲ)有12眼扩增出520bp大小条带,阳性率27.27%;11眼扩增出1004bp大小条带,阳性率25%;520bp兼1004bp条带有3眼阳性率6.81%。以上两组间相互比较结果只有(Ⅰ)组和(Ⅱ)组有显著性差异(X2=5.88、4.66,p0.05)。(Ⅰ)组和(Ⅲ)组(X2=2.77、2.29、0.124、p0.05)、(Ⅱ)组和(Ⅲ)组(X2=2.97、1.76、0.124,p0.05)均无显著性差异。2.非典型急性结膜炎组(Ⅲ)细菌培养法有17眼培养出阳性,阳性率为38.6%与PCR检测法比较无显著性差异(X2=1.77,P0.05)。 结论：1.PCR检测法对非典型的急性结膜炎进行病因诊断具有临床应用意义。 2.虽然PCR检测法阳性检出率较细菌培养法无明显差异,但对样本要求不高,且经济又时间短,对急性结膜炎诊断具有良好的应用前景,值得推广。
[Abstract]:Objective: to investigate the clinical significance of polymerase chain reaction (PCR) in the etiological diagnosis of atypical acute conjunctivitis by DNA extraction and PCR amplification of conjunctival sac secretions from patients with atypical acute conjunctivitis. Methods: 22 eyes of 22 patients with typical acute bacterial conjunctivitis, 22 patients with acute viral conjunctivitis, 23 patients with typical allergic conjunctivitis, 44 patients with atypical acute conjunctivitis and 44 patients with atypical acute conjunctivitis were selected. 22 eyes of 22 normal people had conjunctivitis and the severe side of conjunctivitis was taken. The DNA extracted from conjunctival sac secretion was divided into two parts. Bacterial primer and adenovirus primer were added to amplify PCR twice. The results were observed in the gel imaging analysis system after gel electrophoresis. The results of bacterial culture were observed in patients with atypical acute conjunctivitis at the same time. The result is 1: 1. In typical acute bacterial conjunctivitis group (鈪，

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