miR-183家族在噪声性聋发生发展中的表达及研究

发布时间：2018-05-15 07:56

本文选题：MicroRNA家族 + 听觉丧失　；参考：《临床耳鼻咽喉头颈外科杂志》2014年07期

【摘要】：目的:建立噪声性聋动物模型,并检测不同时间段耳蜗内MicroRNA183家族成员表达量的变化,为探索噪声性聋相关的发病机制提供思路。方法:50只小鼠随机分为5个组(1、7、14、28d实验组及对照组),每组10只。实验组给予中心频率2.0~4.0kHz的窄带噪声,强度为100dB SPL,每天6h,持续3d;对照组不接触噪声。在噪声刺激前及刺激后1、7、14和28d后分别测得ABR阈值。通过耳蜗基膜铺片来检测毛细胞的病理损伤过程,并采用实时定量聚合酶链反应(qRT-PCR)检测MicroRNA183家族成员表达量的变化。采用SPSS17.0软件对数据进行统计学分析。结果:噪声刺激后,各实验组听力与刺激前及对照组相比均明显下降(均P0.01);1d组较其他实验组下降更明显(P0.01),7d组次之(P0.05);14d与28d组间听力下降程度相近,差异无统计学意义(P0.05)。铺片检测发现随着时间的延长,外毛细胞排列发生混乱、变形以及缺损数量逐渐增加,且多集中在第1和第2排,内毛细胞未见明显缺失。qRT-PCR检测显示:噪声刺激后1d和7d组miR-183/96/182的表达量均较对照组下降(均P0.01),而1d与7d组间差异无统计学意义(P0.05);14d组的表达量呈明显上升趋势(P0.01);28d组表达量较14d组明显回降(P0.01),但仍高于1d组和7d组(P0.01)。结论:MicroRNA183家族成员在噪声刺激后的一段时间内表达量存在着显著变化,可能作为特异性基因在噪声性聋发生发展中起着重要作用。
[Abstract]:Objective: to establish an animal model of noise induced deafness, and to detect the changes in the expression of MicroRNA183 family members in the cochlea of different time periods, in order to provide ideas for exploring the pathogenesis of noise related deafness. Methods: 50 mice were randomly divided into 5 groups (1,7,14,28d experimental group and control group), with 10 rats in each group. The experimental group was given a narrow band of central frequency 2.0~4.0kHz. The noise, intensity was 100dB SPL, 6h per day and continuous 3D; the control group was not exposed to noise. The ABR threshold was measured before and after 1,7,14 and 28d after the stimulation of noise. The pathological process of the hair cells was detected by the cochlear membrane sheet laying, and the change of the expression of the MicroRNA183 family members was detected by real-time quantitative polymerase chain reaction (qRT-PCR). The data were statistically analyzed with SPSS17.0 software. Results: after noise stimulation, all the experimental groups were significantly lower than before and before the stimulation and the control group (all P0.01); the 1D group was more obvious than the other experimental groups (P0.01) and 7d group (P0.05); the degree of hearing loss between 14d and 28d group was similar, the difference was not statistically significant (P0.05). With the prolongation of time, the arrangement of outer hair cells was confused, the number of deformable and defect increased gradually, and the first and second rows were concentrated in the first and second rows. The expression of miR-183/96/182 in the group of 1D and 7d was lower than that of the control group (P0.01), but there was no statistical difference between the 1D and the 7d group (P). 0.05) the expression of 14d group showed an obvious upward trend (P0.01), and the expression of 28d group was significantly lower than that of group 14d (P0.01), but it was still higher than that of group 1D and 7d group (P0.01). Conclusion: the expression of the MicroRNA183 family members in a period of time after noise stimulation has a significant change, which can be used as a specific gene in the development of noise induced deafness. Use.

【作者单位】：南昌大学第一附属医院耳鼻咽喉-头颈外科;九江市第三人民医院五官科;
【基金】：江西省自然科学基金项目(No:20124BAB205001)
【分类号】：R764.43

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