视网膜激光光凝对增殖性糖尿病视网膜病变患者视网膜前膜新生血管生成的作用

发布时间：2018-05-16 23:33

本文选题：糖尿病性视网膜病变 + 视网膜激光光凝　；参考：《安徽医科大学学报》2014年07期

【摘要】：目的探讨增殖性糖尿病视网膜病变(PDR)患者视网膜激光治疗对视网膜前膜新生血管的作用。方法收集PDR患者20例20眼,按照玻璃体手术前是否接受过视网膜激光光凝治疗分为光凝组和非光凝组。收集术中剥离的视网膜前膜行苏木精-伊红染色,观察组织病理学改变。应用免疫组织化学染色法检测CD34在新生血管内皮细胞中的表达,比较两组患者视网膜前膜新生血管的密度及单细胞新生血管密度。结果两组患者的人口基线特征和眼部特征比较,差异无统计学意义(P0.05),非光凝组患者视网膜+++级新生血管8眼,光凝组患者为1眼,二者比较差异有统计学意义(P0.01)。组织病理学结果表明,非光凝组增生膜内可见较多散在的毛细血管型新生血管,多数由单个血管内皮细胞构成管腔,部分区域可见较多出血;光凝组增生膜组织变薄,玻璃样变成分较多,新生毛细血管明显减少。免疫组织化学检测证实,新生血管内皮细胞中CD34均呈强阳性表达,光凝组400倍镜下观察,每个视野新生血管密度为(2.18±1.26)个,非光凝组为(10.00±6.15)个,差异有统计学意义(Z=-2.994,P0.05)。结论视网膜激光光凝可抑制PDR患者视网膜前膜新生血管的形成,从而降低玻璃体手术治疗PDR的难度。
[Abstract]:Objective to investigate the effect of retinal laser therapy on retinal preretinal neovascularization in patients with proliferative diabetic retinopathy (PDR). Methods 20 PDR patients (20 eyes) were divided into photocoagulation group and non-photocoagulation group according to whether they received retinal laser photocoagulation before vitrectomy. The preretinal membranes were stained with hematoxylin-eosin and histopathological changes were observed. Immunohistochemical staining was used to detect the expression of CD34 in neovascularization endothelial cells and to compare the density of neovascularization and single cell neovascularization between the two groups. Results there was no significant difference in population baseline and eye characteristics between the two groups. There were 8 eyes of retinal neovascularization in non-photocoagulation group and 1 eye in photocoagulation group. The difference between the two groups was statistically significant (P 0.01). Histopathological results showed that there were more scattered capillary neovascularization in the proliferative membrane of non-photocoagulation group, most of which were composed of a single vascular endothelial cell, and some areas had more bleeding, and the proliferative membrane tissue in the photocoagulation group became thinner. Glass-like changes into more, neovascularization significantly reduced. The positive expression of CD34 in neovascularization endothelial cells was detected by immunohistochemistry. The density of neovascularization was 2.18 卤1.26 per field in photocoagulation group and 10.00 卤6.15 in non-photocoagulation group. The difference was statistically significant. Conclusion the retinal laser photocoagulation can inhibit the formation of retinal preretinal neovascularization in patients with PDR and reduce the difficulty of vitreous surgery in the treatment of PDR.
【作者单位】：安徽医科大学附属省立医院眼科;
【基金】：安徽省科技厅科研计划项目(编号:08020303105)
【分类号】：R779.63

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