EBV-LMP2A和β-catenin蛋白在鼻咽癌中的表达关系及意义

发布时间：2018-05-18 04:26

本文选题：鼻咽肿瘤 + 潜伏膜蛋白A　；参考：《临床与实验病理学杂志》2014年10期

【摘要】：目的探讨EB病毒编码的潜伏膜蛋白2A(latent membrane protein 2A,LMP2A)和β-连环素(β-catenin)在鼻咽癌(nasopharyngeal carcinoma,NPC)中的表达关系及其临床意义。方法应用免疫组化SP法检测LMP2A和β-catenin蛋白在32例鼻咽黏膜慢性炎、56例NPC和18例NPC淋巴结转移灶中的表达,采用Western blot法和免疫荧光细胞化学染色法观察LMP2A和β-catenin蛋白在CNE2-LMP2A细胞及其对照组CNE2-Vector和CNE2细胞中的表达。结果 (1)LMP2A在NPC及其淋巴结转移灶中的阳性率分别为89.3%(50/56)和77.8%(14/18),均明显高于鼻咽黏膜慢性炎(37.5%,12/32)(P均0.01);β-catenin在NPC及其淋巴结转移灶中的异常表达率分别为62.5%(35/56)和83.3%(15/18),均明显高于鼻咽黏膜慢性炎(3.1%,1/32)(P均0.01);LMP2A与β-catenin正常表达呈负相关(rs=-0.391,P0.01);LMP2A和β-catenin蛋白在鼻咽癌T分期、N分期和临床分期组间表达率的差异均有统计学意义(P0.05或P0.01)。(2)CNE2-LMP2A细胞中β-catenin蛋白表达水平明显高于CNE2-Vector和CNE2细胞;CNE2-LMP2A细胞中β-catenin胞质和胞核的表达水平明显高于CNE2-Vector和CNE2(P均0.01)。结论 LMP2A高表达和β-catenin异常表达与鼻咽癌临床生物学行为进展密切相关,其机制可能与LMP2A上调β-catenin蛋白水平并促进其核转位有关。
[Abstract]:Objective to investigate the expression of Epstein-Barr virus (EBV) -encoded latent membrane protein (2A(latent membrane protein _ 2A) and 尾 -catenin (尾 -catenin) in nasopharyngeal carcinoma (NPC) and its clinical significance. Methods Immunohistochemical SP method was used to detect the expression of LMP2A and 尾 -catenin protein in 56 cases of NPC and 18 cases of NPC lymph node metastasis in 32 cases of nasopharyngeal mucosa chronic inflammation and 18 cases of NPC lymph node metastasis. The expression of LMP2A and 尾 -catenin in CNE2-LMP2A cells and its control group, CNE2-Vector and CNE2 cells, was observed by Western blot and immunofluorescence cytochemical staining. Results the positive rates of LMP2A in NPC and lymph node metastases were 89.3 / 56) and 77.8 / 14 / 18, respectively, which were significantly higher than those in chronic nasopharynx mucosal inflammation 37.5 / 32 / 32 / 0. 01; the abnormal expression rates of 尾 -catenin in NPC and lymph node metastases were 62.5 / 3556) and 83.3 / 15 / 18, respectively, which were significantly higher than those in nasopharynx. There was a negative correlation between the normal expression of 尾 -catenin and LMP2A and the normal expression of 尾 -catenin. There was a significant difference in the expression of LMP2A and 尾 -catenin between T staging group and clinical stage group of nasopharyngeal carcinoma (P 0.05) or the expression level of 尾 -catenin protein in P0.01).(2)CNE2-LMP2A cells was significantly higher than that in P0.01).(2)CNE2-LMP2A cell. The expression of 尾 -catenin in cytoplasm and nucleus of CNE2-Vector and CNE2 cells was significantly higher than that of CNE2-Vector and CNE2(P. Conclusion the high expression of LMP2A and abnormal expression of 尾 -catenin are closely related to the progress of clinical biological behavior of nasopharyngeal carcinoma. The mechanism may be related to the up-regulation of 尾 -catenin protein level and the promotion of nuclear translocation by LMP2A.
【作者单位】：广东医学院病理学教研室;四川省宜宾卫生学校;广东省深圳市龙华新区人民医院;
【基金】：国家重点基础研究发展计划(973计划)子项目(2011CB504800)
【分类号】：R739.63

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