增生性玻璃体视网膜疾病患者玻璃体HIF-1a的表达及意义

发布时间：2018-05-18 20:56

  本文选题：增生性玻璃体视网膜疾病 + 缺氧诱导因子-1α　； 参考：《天津医科大学》2011年硕士论文

【摘要】：目的： 1.定量检测增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)、增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)及正常对照组玻璃体缺氧诱导因子-1α(Hypoxia-inducible factor 1α, HIF-1α)的质量浓度,分析HIF-1α的变化规律。 2.分析增生性玻璃体视网膜疾病患者玻璃体HIF-1α表达的影响因素。 3.探讨HIF-1α在增生性玻璃体视网膜疾病发病机制中的作用。 方法： 在常规玻璃体切除术过程中收集玻璃体待测标本,实验组共69例71眼,其中包括PDR组37例391眼和PVR组32例32眼；正常对照组8例8眼,为本院行角膜移植术后供体眼玻璃体标本。采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay, ELISA)检测受检眼玻璃体中HIF-1α的质量浓度。 结果： 1.PDR组、PVR组与正常对照组玻璃体中HIF-1α质量浓度分别为(301.63±23.18)、(284.88±24.42)、(16.38±3.56)mg/L,三组比较差异有统计学意义(F=273.72,P=0.00),其中PDR组玻璃体HIF-1α质量浓度高于正常对照组,差异有统计学意义(t=23.32,P=0.00),PVR组玻璃体HIF-1α质量浓度高于正常对照组,差异有统计学意义(t=21.74,P=0.00),PDR组玻璃体HIF-1α质量浓度高于PVR组,差异有统计学意义(t=3.01,P=0.00)。 2.PDR组Ⅳ期、Ⅴ期、Ⅵ期玻璃体中HIF-1α质量浓度分别为(274.58±30.05)、(298.37±17.34)、(315.94±18.30)mg/L,三期比较差异有统计学意义(F=8.87,P=0.00),其中V期玻璃体HIF-1α质量浓度高于Ⅳ期,差异有统计学意义(t=-2.44,P=0.02),Ⅵ期玻璃体HIF-1α质量浓度高于Ⅴ期,差异有统计学意义(t=2.59,P=0.01),Ⅵ期玻璃体HIF-1α质量浓度高于Ⅳ期,差异有统计学意义(t=4.08,P=0.00)。 3.PVR组C级、D级玻璃体中HIF-1α质量浓度分别为(267.44±22.45)、(295.34±19.33)mg/L,D级玻璃体HIF-1α质量浓度高于C级,差异有统计学意义(t=3.72,P=0.00)。 4.实验组中合并肾病与不合并肾病组玻璃体HIF-1α质量浓度分别为(302.69±21.65)、(292.50±25.44)mg/L,差异无统计学意义(P=0.22)。 5.实验组中合并高血压与不合并高血压组玻璃体HIF-1α质量浓度分别为(293.54±28.03)、(294.40±23.32)mg/L,差异无统计学意义(P=0.89)。 6.实验组玻璃体HIF-1α质量浓度与眼病史之间行线性回归分析,可得到回归方程Y=1.096X+286.613,故增生性玻璃体视网膜疾病患者玻璃体HIF-1α质量浓度与眼病史之间关联性较弱。 7.实验组玻璃体HIF-1α质量浓度与年龄、性别之间无相关性(r=-0.207,P=0.08；r=0.016,P=0.89),与术前空腹血糖水平、糖尿病病程也无相关性(r=-0.159,P=0.33,r=0.012,P=0.94)。 结论： 1.增生性玻璃体视网膜疾病患者玻璃体HIF-1α质量浓度比正常人明显升高,并且HIF-1α质量浓度随眼底病变程度加重而升高。 2.HIF-1α可能在增生性玻璃体视网膜疾病发病机制中起一定作用。
[Abstract]:Objective: 1. The mass concentrations of proliferative diabetic retinopathy (HIF-1 伪) in proliferative diabetic retinopathy (PVR) and hypoxia-inducible factor 1 伪 (HIF-1 伪) in vitreous hypoxia inducible factor 1 伪 (HIF-1 伪) in normal control group and proliferative retinopathy vitreoretinopathy proliferative vitreoretinopathy (PVR) were measured quantitatively, and the changes of HIF-1 伪 were analyzed. 2. To analyze the influencing factors of HIF-1 伪 expression in vitreous body of proliferative vitreoretinopathy patients. 3. To investigate the role of HIF-1 伪 in the pathogenesis of proliferative vitreoretinal diseases. Methods: Vitreous specimens were collected during routine vitrectomy. There were 69 cases (71 eyes) in experimental group, including 37 cases (391 eyes) in PDR group and 32 eyes (32 eyes) in PVR group, and 8 cases (8 eyes) in normal control group. The vitreous specimen of donor eyes after keratoplasty was performed in our hospital. Enzyme linked immunosorbent assay (Elisa) was used to detect the mass concentration of HIF-1 伪 in vitreous of eyes. Results: The mass concentrations of HIF-1 伪 in vitreous of 1.PDR group and normal control group were 301.63 卤23.18 ~ 284.88 卤24.42 mg / L, respectively. The differences among the three groups were statistically significant (P < 0.05). The content of HIF-1 伪 in vitreous of PDR group was higher than that of normal control group, and the content of HIF-1 伪 in vitreous tissue of PDR group was higher than that of normal control group. The content of HIF-1 伪 in vitreous body was significantly higher in HIF-1 group than that in normal control group (P < 0.05), and the concentration of HIF-1 伪 in vitreous body in group B was significantly higher than that in group PVR (P < 0.05), and there was significant difference in the concentration of HIF-1 伪 in vitreous body between the two groups (P < 0.05), and the concentration of HIF-1 伪 in vitreous tissue was significantly higher than that in group PVR (P < 0.05). In 2.PDR group, the concentration of HIF-1 伪 in vitreous of stage 鈪，

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