激光共聚焦显微镜实时动态观察不同微环境中鼻咽癌细胞摄取大黄素差异研究

发布时间：2018-05-19 22:09

本文选题：激光共聚焦显微镜 + 鼻咽癌细胞　；参考：《药物分析杂志》2014年03期

【摘要】：目的:以人鼻咽癌CNE-1、CNE-2细胞为研究对象,采用激光共聚焦显微镜动态检测细胞对大黄素的摄取,探讨不同微环境对CNE-1、CNE-2细胞摄取大黄素的影响。方法:通过MTT方法检测大黄素对CNE-1和CNE-2细胞在乏氧和正常培养的条件下的生长抑制作用,选择无细胞毒性浓度的大黄素,激光共聚焦显微镜完美对焦系统分别观察两种细胞在乏氧、无血清、与血管内皮细胞共培养后对大黄素的摄取及这些过程的细胞形态学变化。结果:鼻咽癌2种细胞无论单独培养和共培养,在乏氧及无血清条件下摄取大黄素后,与相应的对照组细胞比较荧光强度最强,达到最大摄取浓度所需时间最短(P0.05);总体而言,CNE-2细胞对大黄素的摄取速度快于CNE-1细胞,共培养体系中CNE-1细胞对大黄素的摄取速度慢于单细胞培养体系,而CNE-2细胞则相反。乏氧状态的鼻咽癌细胞在摄取大黄素后会出现明显的细胞皱缩、变圆、起泡等凋亡的形态学变化。大黄素对鼻咽癌CNE-1和CNE-2细胞在乏氧和不乏氧情况下半数抑制浓度(IC50)分别为18.34、16.87μg·mL-1和20.44、19.87μg·mL-1。结论:鼻咽癌细胞对大黄素的摄取与细胞的培养条件、乏氧状态等微环境有关,改变微环境有利于提高药物的靶向性。
[Abstract]:Aim: to investigate the effect of different microenvironment on emodin uptake in CNE-1nCNE-2 cells of human nasopharyngeal carcinoma (NPC) by laser confocal microscopy (LSCM). Methods: the inhibitory effects of emodin on the growth of CNE-1 and CNE-2 cells under hypoxia and normal culture were detected by MTT method. Emodin with no cytotoxic concentration was selected. The uptake of emodin and the morphological changes of these processes were observed by laser scanning confocal microscopy (LSCM) in hypoxic, serum-free and co-cultured vascular endothelial cells (VEC). Results: the fluorescence intensity of two kinds of nasopharyngeal carcinoma cells was stronger than that of the control group after emodin was ingested under hypoxia and serum free condition, whether cultured alone or co-cultured. In general, the uptake rate of emodin by CNE-1 cells was faster than that of CNE-1 cells, and the uptake rate of emodin by CNE-1 cells was slower than that by monocyte culture system, while that of CNE-2 cells was opposite in co-culture system. Anoxic nasopharyngeal carcinoma cells had obvious morphological changes of apoptosis, such as shrinkage, roundness and bubbling after emodin uptake. The IC50 of emodin on nasopharyngeal carcinoma CNE-1 and CNE-2 cells was 18.34 渭 g mL-1 and 20.44 渭 g ml ~ (-1), respectively. Conclusion: the uptake of emodin in nasopharyngeal carcinoma cells is related to cell culture conditions, hypoxia status and other microenvironments.
【作者单位】：广西医科大学;
【基金】：国家自然基金项目(No.81060270) 广西医学科学实验中心开放基金(No.KFJJ2011-25)
【分类号】：R739.63

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