布地奈德预防应用对变应性鼻炎大鼠模型IL-4和IL-5表达影响的研究

发布时间：2018-05-20 12:22

本文选题：鼻炎 + 变应性　；参考：《临床耳鼻咽喉头颈外科杂志》2014年14期

【摘要】：目的:探讨布地奈德预防性应用对变应性鼻炎最轻持续炎症反应大鼠模型鼻黏膜IL-4、IL-5表达的影响。方法:80只健康SD大鼠,雌雄不限,随机分为变应性鼻炎组(A组,20只)、实验组(B组,20只)、对照组(C组,20只)、空白对照组(D组)。A组制作变应性鼻炎模型,B、C组制作变应性鼻炎最轻持续炎症反应模型,模型建立随后处死一半A、B、C和D组大鼠,观察鼻黏膜嗜酸粒细胞(EOS)浸润和ICAM-1、IL-4、IL-5的表达情况。剩余B组大鼠用布地奈德(64μg,2次/d,每次每侧鼻孔1喷)治疗2周,剩余A、C、D组大鼠用生理盐水喷鼻,2周后3组大鼠均用1%卵清蛋白滴鼻,1次/d,共1周,激发完成后处死全部大鼠,观察鼻黏膜EOS和IL-4、IL-5表达情况。结果:布地奈德药物治疗后,B组平均EOS计数与A、C组相比,差异有统计学意义(P0.05);B组与D组相比,差异无统计学意义(P0.05)。B组IL-4灰度值与A、C组相比,差异有统计学意义(P0.05);A组与C组相比,差异无统计学意义(P0.05);鼻黏膜组织中IL-5阳性区的灰度值比较结果亦同上。结论:布地奈德最轻持续炎症反应期应用可显著抑制变应。
[Abstract]:Aim: to investigate the effect of budesonide prophylactic application on the expression of IL-4 and IL-5 in nasal mucosa of allergic rhinitis rats. Methods 80 healthy Sprague-Dawley rats, male and female, They were randomly divided into two groups: group A (n = 20), group B (n = 20), group C (n = 20), group D (n = 20), group A (n = 20), and group B (n = 20). After the establishment of the model, half of the rats in group A and D were killed, and the infiltration of eosinophils and the expression of IL-4 IL-5 in nasal mucosa were observed. The rats in the remaining group B were treated with budesonide 64 渭 g / d twice a day (1 nasal cavity per nostril) for 2 weeks. After 2 weeks of nasal spray with normal saline, all the rats in the 3 groups were treated with 1% ovalbumin nasal drops for 1 d / d for 1 week. All the rats were killed after stimulation. The expression of IL-5 and EOS in nasal mucosa was observed. Results: after budesonide drug therapy, the average EOS count in group B was significantly different from that in group A (P 0.05). There was no significant difference between group B and group D, and there was no significant difference in the gray value of IL-4 between group B and group A (P 0.05), and there was significant difference between group A (P 0.05) and group C (P 0.05), and there was no significant difference between group B (P 0.05) and group C (P 0.05). The difference was not statistically significant (P 0.05), and the gray value of IL-5 positive area in nasal mucosa was the same as that in nasal mucosa. Conclusion: budesonide can significantly inhibit allergic reaction in the least sustained inflammatory response period.
【作者单位】：郑州大学第二附属医院耳鼻咽喉科;
【分类号】：R765.21

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